Back to Search
Start Over
Identification of a plastid intercistronic expression element (IEE) facilitating the expression of stable translatable monocistronic mRNAs from operons.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2007 Dec; Vol. 52 (5), pp. 961-72. Date of Electronic Publication: 2007 Sep 06. - Publication Year :
- 2007
-
Abstract
- Most plastid genes are part of operons and expressed as polycistronic mRNAs. Many primary polycistronic transcripts undergo post-transcriptional processing in monocistronic or oligocistronic units. At least some polycistronic transcripts are not translatable, and endonucleolytic processing may therefore be a prerequisite for translation to occur. As the requirements for intercistronic mRNA processing into stable monocistronic transcript are not well understood, we have sought to define minimum sequence elements that trigger processing and thus are capable of generating stable translatable monocistronic mRNAs. We describe here the in vivo identification of a small intercistronic expression element that mediates intercistronic cleavage into stable monocistronic transcripts. Separation of foreign genes by this element facilitates transgene stacking in operons, and thus will help to expand the range of applications of transplastomic technology.
- Subjects :
- Base Sequence
Chromosome Mapping
DNA, Intergenic chemistry
Gene Expression
Genes, Reporter
Molecular Sequence Data
Plants, Genetically Modified metabolism
Sequence Alignment
Sequence Analysis, RNA
Operon
Plastids genetics
RNA, Messenger metabolism
Regulatory Elements, Transcriptional
Nicotiana genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0960-7412
- Volume :
- 52
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 17825052
- Full Text :
- https://doi.org/10.1111/j.1365-313X.2007.03261.x