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Ezrin and alpha-smooth muscle actin are immunohistochemical prognostic markers in conventional osteosarcomas.
- Source :
-
Virchows Archiv : an international journal of pathology [Virchows Arch] 2007 Dec; Vol. 451 (6), pp. 999-1007. Date of Electronic Publication: 2007 Sep 05. - Publication Year :
- 2007
-
Abstract
- Ezrin is a cytoskeleton linker protein that is actively involved in the metastatic process of cancer cells. We have searched for a prognostic value of ezrin and some of its partners: alpha-smooth muscle actin and CD44H in 37 patients with an osteosarcoma. Automate immunohistochemistry (IHC) with anti-ezrin, alpha-smooth muscle actin and CD44H antibodies was performed in 66 specimens: 37 biopsies before chemotherapy, 16 resected tumours of "poor" responders and 13 metastases. The messenger RNA (mRNA) levels of ezrin of 13 frozen biopsies and 4 metastases were evaluated by real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR). All results were correlated to the following clinical data. Ezrin expression by IHC was found in 62% of 37 biopsies in the different histological subtypes. A good correlation was found between positive or negative samples by IHC and mRNA levels. Ezrin expression was recorded in 84.5% of metastastic samples. The mean expression of ezrin was higher in metastases than biopsies (p = 0.024). In multivariate analysis, ezrin was an independent prognostic marker for event-free survival and overall survival (OS) with p < 0.001 and p = 0.003, respectively, and alpha-smooth muscle actin for OS only (p = 0.024). Our findings suggest that ezrin and alpha-smooth muscle actin are predictive IHC prognostic markers for patients with an osteosarcoma.
- Subjects :
- Actins genetics
Biomarkers, Tumor metabolism
Bone Neoplasms mortality
Bone Neoplasms pathology
Bone Neoplasms therapy
Combined Modality Therapy
Cytoskeletal Proteins genetics
Female
France epidemiology
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Male
Middle Aged
Muscle, Smooth metabolism
Neoplasm Metastasis pathology
Osteosarcoma mortality
Osteosarcoma secondary
Osteosarcoma therapy
RNA, Messenger metabolism
Survival Rate
Actins metabolism
Bone Neoplasms metabolism
Cytoskeletal Proteins metabolism
Osteosarcoma metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0945-6317
- Volume :
- 451
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Virchows Archiv : an international journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 17786474
- Full Text :
- https://doi.org/10.1007/s00428-007-0474-8