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Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 1991 Sep; Vol. 29 (9), pp. 2056-8. - Publication Year :
- 1991
-
Abstract
- A competitive inhibition enzyme-linked immunosorbent assay (CI ELISA) was developed to detect antibody to Babesia equi. One hundred fifty-four equine serum samples from 19 countries were tested for antibody to B. equi by the complement fixation test and by CI ELISA. The CI ELISA and complement fixation test results agreed in 94% (144) of the serum samples tested. The 10 discrepant serum samples were retested and analyzed for ability to immunoprecipitate in vitro translation products from B. equi merozoite mRNA. Five discrepant results were clearly resolved in favor of the CI ELISA, and the remaining five discrepancies were not definitively resolved.
- Subjects :
- Animals
Antibodies, Monoclonal
Babesiosis diagnosis
Babesiosis immunology
Binding, Competitive
Complement Fixation Tests
Evaluation Studies as Topic
Horse Diseases diagnosis
Horse Diseases immunology
Horses
Protozoan Proteins immunology
Antibodies, Protozoan analysis
Babesia immunology
Enzyme-Linked Immunosorbent Assay methods
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 29
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 1774334
- Full Text :
- https://doi.org/10.1128/jcm.29.9.2056-2058.1991