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Surface plasmon resonance immunosensor for histamine based on an indirect competitive immunoreaction.
- Source :
-
Analytica chimica acta [Anal Chim Acta] 2006 Aug 18; Vol. 576 (1), pp. 77-83. Date of Electronic Publication: 2006 Mar 03. - Publication Year :
- 2006
-
Abstract
- The use of a surface plasmon resonance immunosensor for the analysis of histamine (beta-imidazole ethylamine) is described. The method is based on an indirect competitive reaction of an anti-histamine antibody in a sample solution with histamine immobilized on a sensor chip and with histamine in the sample solution. A sensor chip immobilized with histamine was prepared using a self-assembly monolayer of 11-mercaptoundecanoic acid (11-MUA) as an anchor membrane, followed by an amino-coupling reaction with histamine after activation of the 11-MUA layer on the sensor chip by treatment with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydroxysuccinimide. The sensor chip can be reused, after regeneration with a 10mM HCl solution, which dissociates the anti-histamine antibody complex from histamine on the sensor chip. The affinity constants for the immunocomplex of the anti-histamine antibody with histamine in the solution and for that of the anti-histamine antibody with histamine immobilized on the sensor chip were calculated to be 1.5 x 10(7) and 7.2 x 10(5) M(-1), respectively, by assuming a Langmuir-type adsorption of the anti-histamine antibody to histamine immobilized on the sensor chip. The detection limit of the method was determined to be 3ppb.
Details
- Language :
- English
- ISSN :
- 1873-4324
- Volume :
- 576
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Analytica chimica acta
- Publication Type :
- Academic Journal
- Accession number :
- 17723617
- Full Text :
- https://doi.org/10.1016/j.aca.2006.01.078