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Surface plasmon resonance immunosensor for histamine based on an indirect competitive immunoreaction.

Authors :
Li Y
Kobayashi M
Furui K
Soh N
Nakano K
Imato T
Source :
Analytica chimica acta [Anal Chim Acta] 2006 Aug 18; Vol. 576 (1), pp. 77-83. Date of Electronic Publication: 2006 Mar 03.
Publication Year :
2006

Abstract

The use of a surface plasmon resonance immunosensor for the analysis of histamine (beta-imidazole ethylamine) is described. The method is based on an indirect competitive reaction of an anti-histamine antibody in a sample solution with histamine immobilized on a sensor chip and with histamine in the sample solution. A sensor chip immobilized with histamine was prepared using a self-assembly monolayer of 11-mercaptoundecanoic acid (11-MUA) as an anchor membrane, followed by an amino-coupling reaction with histamine after activation of the 11-MUA layer on the sensor chip by treatment with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydroxysuccinimide. The sensor chip can be reused, after regeneration with a 10mM HCl solution, which dissociates the anti-histamine antibody complex from histamine on the sensor chip. The affinity constants for the immunocomplex of the anti-histamine antibody with histamine in the solution and for that of the anti-histamine antibody with histamine immobilized on the sensor chip were calculated to be 1.5 x 10(7) and 7.2 x 10(5) M(-1), respectively, by assuming a Langmuir-type adsorption of the anti-histamine antibody to histamine immobilized on the sensor chip. The detection limit of the method was determined to be 3ppb.

Details

Language :
English
ISSN :
1873-4324
Volume :
576
Issue :
1
Database :
MEDLINE
Journal :
Analytica chimica acta
Publication Type :
Academic Journal
Accession number :
17723617
Full Text :
https://doi.org/10.1016/j.aca.2006.01.078