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Cholesterol accumulation is increased in macrophages of phospholipid transfer protein-deficient mice: normalization by dietary alpha-tocopherol supplementation.
- Source :
-
Arteriosclerosis, thrombosis, and vascular biology [Arterioscler Thromb Vasc Biol] 2007 Nov; Vol. 27 (11), pp. 2407-12. Date of Electronic Publication: 2007 Aug 23. - Publication Year :
- 2007
-
Abstract
- Objective: Phospholipid transfer protein (PLTP) is a multifunctional, extracellular lipid transport protein that plays a major role in lipoprotein metabolism and atherosclerosis. Recent in vivo studies suggested that unlike systemic PLTP, macrophage-derived PLTP would be antiatherogenic. The present study aimed at characterizing the atheroprotective properties of macrophage-derived PLTP.<br />Methods and Results: Peritoneal macrophages were isolated from PLTP-deficient and wild-type mice and their biochemical characteristics were compared. It is shown that macrophages isolated from PLTP-deficient mice have increased basal cholesterol content and accumulate more cholesterol in the presence of LDL compared with wild-type cells. Cholesterol parameters in macrophages of PLTP-deficient mice were normalized by dietary alpha-tocopherol supplementation.<br />Conclusions: The antiatherogenic properties of macrophage-derived PLTP are related at least in part to its ability to reduce cholesterol accumulation in macrophages through changes in the alpha-tocopherol content and oxidative status of the cells.
- Subjects :
- Animals
Lipoproteins, LDL metabolism
Malondialdehyde metabolism
Mice
Mice, Knockout
Cholesterol metabolism
Dietary Supplements
Macrophages, Peritoneal drug effects
Macrophages, Peritoneal metabolism
Phospholipid Transfer Proteins deficiency
Vitamins pharmacology
alpha-Tocopherol pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1524-4636
- Volume :
- 27
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Arteriosclerosis, thrombosis, and vascular biology
- Publication Type :
- Academic Journal
- Accession number :
- 17717294
- Full Text :
- https://doi.org/10.1161/ATVBAHA.107.151753