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BubR1 and APC/EB1 cooperate to maintain metaphase chromosome alignment.
- Source :
-
The Journal of cell biology [J Cell Biol] 2007 Aug 27; Vol. 178 (5), pp. 773-84. Date of Electronic Publication: 2007 Aug 20. - Publication Year :
- 2007
-
Abstract
- The accurate segregation of chromosomes in mitosis requires the stable attachment of microtubules to kinetochores. The details of this complex and dynamic process are poorly understood. In this study, we report the interaction of a kinetochore-associated mitotic checkpoint kinase, BubR1, with two microtubule plus end-associated proteins, adenomatous polyposis coli (APC) and EB1, providing a potential link in stable kinetochore microtubule attachment. Using immunodepletion from and antibody addition to Xenopus laevis egg extracts, we show that BubR1 and its kinase activity are essential for positioning chromosomes at the metaphase plate. BubR1 associates with APC and EB1 in egg extracts, and the complex formation is necessary for metaphase chromosome alignment. Using purified components, BubR1 directly phosphorylates APC and forms a ternary complex with APC and microtubules. These findings support a model in which BubR1 kinase may directly regulate APC function involved in stable kinetochore microtubule attachment.
- Subjects :
- Adenomatous Polyposis Coli Protein genetics
Animals
Cell Cycle Proteins
Humans
Kinetochores metabolism
Microtubule-Associated Proteins genetics
Microtubules metabolism
Mitosis physiology
Multiprotein Complexes
Oocytes cytology
Oocytes physiology
Protein Kinases genetics
Xenopus Proteins genetics
Xenopus laevis genetics
Adenomatous Polyposis Coli Protein metabolism
Chromosomes metabolism
Metaphase physiology
Microtubule-Associated Proteins metabolism
Protein Kinases metabolism
Xenopus Proteins metabolism
Xenopus laevis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 178
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 17709426
- Full Text :
- https://doi.org/10.1083/jcb.200702138