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3,3'-Diindolylmethane stimulates murine immune function in vitro and in vivo.
- Source :
-
The Journal of nutritional biochemistry [J Nutr Biochem] 2008 May; Vol. 19 (5), pp. 336-44. Date of Electronic Publication: 2007 Aug 17. - Publication Year :
- 2008
-
Abstract
- 3,3'-Diindolylmethane (DIM), a major condensation product of indole-3-carbinol, exhibits chemopreventive properties in animal models of cancer. Recent studies have shown that DIM stimulates interferon-gamma (IFN-gamma) production and potentiates the IFN-gamma signaling pathway in human breast cancer cells via a mechanism that includes increased expression of the IFN-gamma receptor. The goal of this study was to test the hypothesis that DIM modulates the murine immune function. Specifically, the effects of DIM were evaluated in a panel of murine immune function tests that included splenocyte proliferation, reactive oxygen species (ROS) generation, cytokine production and resistance to viral infection. DIM was found to induce proliferation of splenocytes as well as augment mitogen- and interleukin (IL)-2-induced splenocyte proliferation. DIM also stimulated the production of ROS by murine peritoneal macrophage cultures. Oral administration of DIM, but not intraperitoneal injection, induced elevation of serum cytokines in mice, including IL-6, granulocyte colony-stimulating factor (G-CSF), IL-12 and IFN-gamma. Finally, in a model of enteric virus infection, oral DIM administration to mice enhanced both clearance of reovirus from the GI tract and the subsequent mucosal IgA response. Thus, DIM is a potent stimulator of immune function. This property might contribute to the cancer inhibitory effects of this indole.
- Subjects :
- Animals
Antibodies, Viral analysis
Cell Proliferation drug effects
Cells, Cultured
Cytokines biosynthesis
Female
Gene Expression Regulation drug effects
Immunoglobulin A drug effects
Immunoglobulin A immunology
Intestinal Mucosa drug effects
Intestinal Mucosa immunology
Macrophages, Peritoneal cytology
Macrophages, Peritoneal drug effects
Macrophages, Peritoneal immunology
Macrophages, Peritoneal metabolism
Male
Mice
Mice, Inbred C57BL
Orthoreovirus, Mammalian drug effects
Orthoreovirus, Mammalian immunology
Reactive Oxygen Species metabolism
Reoviridae Infections immunology
Spleen cytology
Spleen immunology
Anticarcinogenic Agents pharmacology
Immunity drug effects
Indoles pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0955-2863
- Volume :
- 19
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of nutritional biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 17707631
- Full Text :
- https://doi.org/10.1016/j.jnutbio.2007.05.004