Back to Search
Start Over
Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2007 Sep 15; Vol. 196 (6), pp. 936-44. Date of Electronic Publication: 2007 Aug 06. - Publication Year :
- 2007
-
Abstract
- Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty-two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype 1 and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak-associated serotypes of S. pneumoniae.
- Subjects :
- Alleles
Bacterial Proteins biosynthesis
Bacterial Proteins genetics
Bacterial Proteins toxicity
Cluster Analysis
DNA, Bacterial chemistry
DNA, Bacterial genetics
Genotype
Hemolysis
Humans
Molecular Sequence Data
Polymorphism, Genetic
Sequence Analysis, DNA
Sequence Homology
Serotyping
Streptococcus pneumoniae classification
Streptococcus pneumoniae genetics
Streptolysins genetics
Streptolysins toxicity
Virulence Factors genetics
Virulence Factors toxicity
Disease Outbreaks
Pneumococcal Infections epidemiology
Pneumococcal Infections microbiology
Streptococcus pneumoniae metabolism
Streptolysins biosynthesis
Virulence Factors biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1899
- Volume :
- 196
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 17703426
- Full Text :
- https://doi.org/10.1086/520091