Back to Search
Start Over
FGF-2 release from the lens capsule by MMP-2 maintains lens epithelial cell viability.
- Source :
-
Molecular biology of the cell [Mol Biol Cell] 2007 Nov; Vol. 18 (11), pp. 4222-31. Date of Electronic Publication: 2007 Aug 15. - Publication Year :
- 2007
-
Abstract
- The lens is an avascular tissue, separated from the aqueous and vitreous humors by its own extracellular matrix, the lens capsule. Here we demonstrate that the lens capsule is a source of essential survival factors for lens epithelial cells. Primary and immortalized lens epithelial cells survive in low levels of serum and are resistant to staurosporine-induced apoptosis when they remain in contact with the lens capsule. Physical contact with the capsule is required for maximal resistance to stress. The lens capsule is also a source of soluble factors including fibroblast growth factor 2 (FGF-2) and perlecan, an extracellular matrix component that enhances FGF-2 activity. Matrix metalloproteinase 2 (MMP-2) inhibition as well as MMP-2 pretreatment of lens capsules greatly reduced the protective effect of the lens capsule, although this could be largely reversed by the addition of either conditioned medium or recombinant FGF-2. These data suggest that FGF-2 release from the lens capsule by MMP-2 is essential to lens epithelial cell viability and survival.
- Subjects :
- Apoptosis Regulatory Proteins metabolism
Cell Adhesion
Cell Line
Cell Survival drug effects
Culture Media, Serum-Free
Epithelial Cells drug effects
Epithelial Cells metabolism
Gene Expression Regulation, Enzymologic
Humans
Matrix Metalloproteinase 2 genetics
Microscopy, Electron
Solubility
Staurosporine pharmacology
Epithelial Cells cytology
Fibroblast Growth Factor 2 metabolism
Lens Capsule, Crystalline cytology
Lens Capsule, Crystalline metabolism
Matrix Metalloproteinase 2 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1059-1524
- Volume :
- 18
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Molecular biology of the cell
- Publication Type :
- Academic Journal
- Accession number :
- 17699594
- Full Text :
- https://doi.org/10.1091/mbc.e06-05-0416