Back to Search
Start Over
Expression analysis and RNA localization of PAI-RBP1 (SERBP1) in epithelial ovarian cancer: association with tumor progression.
- Source :
-
Gynecologic oncology [Gynecol Oncol] 2007 Nov; Vol. 107 (2), pp. 266-73. Date of Electronic Publication: 2007 Aug 14. - Publication Year :
- 2007
-
Abstract
- Objective: The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. A recently characterized protein, PAI-RBP1 (PAI-1 mRNA Binding Protein 1), appears to regulate the stability of PAI-1 mRNA. Expression of PAI-RBP1 (the new, approved gene symbol is SERBP1) has not been previously analyzed in human tumors. We present herein for the first time expression analysis of PAI-RBP1 in epithelial ovarian cancer.<br />Methods: PAI-RBP1 was identified as gene overexpressed in ovarian cancer by an in silico approach using EST database mining. A thorough expression analysis of PAI-RBP1 and PAI-1 was performed in normal ovary (n=4), benign (n=6) and malignant (n=42) ovarian lesions using non-radioactive RNA in situ hybridization and immunohistochemistry, respectively.<br />Results: PAI-RBP1 mRNA and PAI-1 were significantly overexpressed in tumor epithelial cells as compared to benign and normal ovarian tissue. A significant correlation between PAI-RBP1 expression and advanced disease stage (FIGO) was found (p=0.042).<br />Conclusion: In ovarian cancer, PAI-RBP1 is significantly overexpressed in tumor epithelial cells, suggesting a biological role in tumor invasion and metastasis. Its expression is higher in advanced disease, thus the prognostic significance of PAI-RBP1 in ovarian cancer remains to be evaluated in further studies.
- Subjects :
- Adult
Aged
Aged, 80 and over
Analysis of Variance
Biomarkers, Tumor genetics
Carcinoma mortality
Disease Progression
Female
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
In Situ Hybridization
Lymphatic Metastasis
Middle Aged
Neoplasm Staging
Neoplasm, Residual
Ovarian Neoplasms mortality
Plasminogen Activator Inhibitor 1 genetics
Predictive Value of Tests
Prognosis
RNA, Messenger analysis
RNA, Neoplasm analysis
Retinol-Binding Proteins, Cellular genetics
Survival Analysis
Up-Regulation
Biomarkers, Tumor analysis
Carcinoma chemistry
Carcinoma pathology
Ovarian Neoplasms chemistry
Ovarian Neoplasms pathology
Plasminogen Activator Inhibitor 1 analysis
Retinol-Binding Proteins, Cellular analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1095-6859
- Volume :
- 107
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Gynecologic oncology
- Publication Type :
- Academic Journal
- Accession number :
- 17698176
- Full Text :
- https://doi.org/10.1016/j.ygyno.2007.06.023