[TNF-alpha induced reversal of multidrug resistance in human hepatocellular carcinoma cells].

Authors :: Wang Q
Chen XP
Hai S
Li GP
Guan J
Zhang BX
Zhang WG
Source :: Zhonghua wai ke za zhi [Chinese journal of surgery] [Zhonghua Wai Ke Za Zhi] 2007 May 01; Vol. 45 (9), pp. 602-4.
Publication Year :: 2007
Abstract: Objective: To investigate the reversal of multidrug resistance in the cell line HepG2/ADM induced by TNF-alpha.<br />Methods: HepG2/ADM cells were incubated with different concentrations of TNF-alpha (100, 500 and 2500 U/ml) for 72 h. Real-time PCR was performed to compare the mRNA levels of MDR1 with PPAR-alpha in the different concentrations of TNF-alpha treated cells. The Annexin V assay was used to check cell apoptosis induced by 0.5 mg/L adriamycin. Rhodamine 123 efflux assay and MTT assay were used to study P-gp activity and drug resistance in each group, respectively.<br />Results: TNF-alpha could induce down-regulation of MDR1 and up-regulation of PPAR-alpha. Meanwhile, it could enhance cell cytotoxicity and cell apoptosis induced by 0.5 mg/L adriamycin.<br />Conclusions: TNF-alpha could partially reverse the multidrug resistance of HepG2/ADM cells by down-regulating the expression of MDR1 and up-regulating the expression of PPAR-alpha.

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