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Localization of transforming growth factor-alpha in adult Syrian hamster tissues.
- Source :
-
Lymphokine and cytokine research [Lymphokine Cytokine Res] 1991 Oct; Vol. 10 (5), pp. 385-90. - Publication Year :
- 1991
-
Abstract
- Using the cheek pouch of the Syrian hamster as an experimental model for oral carcinogenesis, it has been shown that the expression of transforming growth factor-alpha (TGF-alpha) is consistently associated with the malignant transformation process. We have recently shown that production of TGF-alpha has been localized to normal hamster oral epithelium and bone marrow eosinophils. In this study we investigated the production of this cytokine in other normal hamster adult tissues. By using an EGF-radioreceptor assay, immunohistochemistry, Northern blot analysis, and in situ hybridization we have now further detected the presence of TGF-alpha mRNA and/or protein in the kidney, stomach, and pancreas of normal adult hamster. Together with the previously reported detection of TGF-alpha in oral mucosa and bone marrow eosinophils, these adult normal tissue/cellular sources can serve as sites of TGF-alpha production. The availability of hamster specific reagents (cDNA and monoclonal antibodies) and the delineation of the various adult tissues that could produce TGF-alpha make the Syrian hamster a suitable model for the study of how this multifunctional cytokine can influence normal and pathological processes.
- Subjects :
- Animals
Antibodies, Monoclonal
Cricetinae
Immunohistochemistry
Islets of Langerhans anatomy & histology
Kidney Tubules, Proximal anatomy & histology
Mesocricetus
Nucleic Acid Hybridization
RNA, Messenger analysis
Radioligand Assay
Transforming Growth Factor alpha genetics
Islets of Langerhans chemistry
Kidney Tubules, Proximal chemistry
Stomach chemistry
Transforming Growth Factor alpha analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1056-5477
- Volume :
- 10
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Lymphokine and cytokine research
- Publication Type :
- Academic Journal
- Accession number :
- 1768741