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Detection of equine herpesviruses in aborted foetuses by consensus PCR.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2008 Jan 01; Vol. 126 (1-3), pp. 20-9. Date of Electronic Publication: 2007 Jun 28. - Publication Year :
- 2008
-
Abstract
- The major role of EHV-1 in equine abortion is widely reported in the literature but the contribution of EHV-2, EHV-3, EHV-4 or EHV-5 remains less well documented. The objective of this study is to evaluate the contribution of these five different EHVs to equine abortion in a variety of biological tissues using a consensus polymerase chain reaction (PCR). The test was validated for specificity and sensitivity in horses before screening specimens from 407 foetuses, stillbirths and premature foals collected over a 2.5-year interval. Positive results obtained with this assay were compared to other EHV type-specific PCR or by sequencing. EHV-1 was identified as the major cause of abortion in French mares (59/407 cases). However, there was evidence to suggest some variation in the potential of EHV-1 strains to induce abortion. Indeed, DNA samples from EHV-2 (in three cases) and EHV-5 (in one case) inferred a role of these viruses in abortion. The presence of viral DNA from EHV-3 or EHV-4 strains was not detected in the specimens studied. The data obtained suggest that the consensus herpesvirus PCR is an efficient screening tool. In association with a specific PCR, the test provides a rapid identification of the type of herpesvirus involved in abortion and is useful for routine diagnostic tests as it allows the identification of herpesviruses other than the EHV-1 strain.
- Subjects :
- Abortion, Veterinary diagnosis
Animals
Base Sequence
Female
Herpesviridae Infections diagnosis
Herpesviridae Infections virology
Horse Diseases diagnosis
Horses
Male
Molecular Sequence Data
Reproducibility of Results
Sensitivity and Specificity
Stillbirth veterinary
Varicellovirus classification
Varicellovirus genetics
Aborted Fetus virology
Abortion, Veterinary virology
Herpesviridae Infections veterinary
Horse Diseases virology
Polymerase Chain Reaction veterinary
Varicellovirus isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1135
- Volume :
- 126
- Issue :
- 1-3
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 17686590
- Full Text :
- https://doi.org/10.1016/j.vetmic.2007.06.019