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[Molecular forms of alpha-1-acid glycoprotein in human blood serum. Differences in levels of di-, tri-, and tetra-antennary N-linked carbohydrate chains].
- Source :
-
Bioorganicheskaia khimiia [Bioorg Khim] 1991 May; Vol. 17 (5), pp. 663-70. - Publication Year :
- 1991
-
Abstract
- alpha 1-Acid glycoprotein isolated from healthy individuals blood was separated on Con A-Sepharose into three fractions: non-bound (AGP-1, 84%, 43.5 kDa), Con A-bound (AGP-2, 14%, 41.3 kDa), and Con A-tightly bound (AGP-3, 2%, 39.6 kDa). Amino acid compositions of these fractions were similar but carbohydrate ones differed. HPLC analysis of 7-amino-4-methylcoumarin derivatives of the oligosaccharides in combination with their sequential exoglycosidase digestion showed that AGP-1, AGP-2, and AGP-3 have the same set of oligosaccharides and differ only by their proposition. A minor quantity of agalacto-oligosaccharides (with a terminal GlcNAc residue) was identified.
Details
- Language :
- Russian
- ISSN :
- 0132-3423
- Volume :
- 17
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Bioorganicheskaia khimiia
- Publication Type :
- Academic Journal
- Accession number :
- 1768292