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Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells.

Authors :
Mercer N
Ahmed H
Etcheverry SB
Vasta GR
Cortizo AM
Source :
Molecular and cellular biochemistry [Mol Cell Biochem] 2007 Dec; Vol. 306 (1-2), pp. 87-94. Date of Electronic Publication: 2007 Jul 28.
Publication Year :
2007

Abstract

Advanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients.

Details

Language :
English
ISSN :
0300-8177
Volume :
306
Issue :
1-2
Database :
MEDLINE
Journal :
Molecular and cellular biochemistry
Publication Type :
Academic Journal
Accession number :
17660952
Full Text :
https://doi.org/10.1007/s11010-007-9557-8