Back to Search
Start Over
Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells.
- Source :
-
Molecular and cellular biochemistry [Mol Cell Biochem] 2007 Dec; Vol. 306 (1-2), pp. 87-94. Date of Electronic Publication: 2007 Jul 28. - Publication Year :
- 2007
-
Abstract
- Advanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients.
- Subjects :
- Animals
Cell Differentiation
Cells, Cultured
Fluorescent Antibody Technique
Galectin 3 genetics
Galectin 3 metabolism
Mice
Osteoblasts metabolism
Receptor for Advanced Glycation End Products
Receptors, Immunologic genetics
Reverse Transcriptase Polymerase Chain Reaction
Apoptosis
Glycation End Products, Advanced pharmacology
Osteoblasts drug effects
Receptors, Immunologic metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0300-8177
- Volume :
- 306
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Molecular and cellular biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 17660952
- Full Text :
- https://doi.org/10.1007/s11010-007-9557-8