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Spontaneous and prostatic steroid binding protein peptide-induced autoimmune prostatitis in the nonobese diabetic mouse.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2007 Aug 01; Vol. 179 (3), pp. 1559-67. - Publication Year :
- 2007
-
Abstract
- Chronic nonbacterial prostatitis is a poorly defined syndrome of putative autoimmune origin. To further understand its pathogenesis, we have analyzed autoimmune prostatitis in the NOD mouse, a strain genetically prone to develop different organ-specific autoimmune diseases. Spontaneous development of autoimmune prostatitis in the NOD male, defined by lymphomonuclear cell infiltration in the prostate gland, is well-established by approximately 20 wk of age and is stably maintained afterward. Disease development is indistinguishable in NOD and NOR mice, but is markedly delayed in IFN-gamma-deficient NOD mice. A T cell response to the prostate-specific autoantigen prostatic steroid-binding protein (PSBP) can be detected in NOD males before development of prostate infiltration, indicating lack of tolerance to this self Ag. The intraprostatic inflammatory infiltrate is characterized by Th1-type CD4(+) T cells, which are able to transfer autoimmune prostatitis into NOD.SCID recipients. We characterize here experimental autoimmune prostatitis, detected by intraprostatic infiltrate and PSBP-specific T cell responses, induced in 6- to 8-wk-old NOD males by immunization with synthetic peptides corresponding to the C1 subunit of PSBP. Three PSBP peptides induce in NOD mice vigorous T and B cell responses, paralleled by a marked lymphomononuclear cell infiltration in the prostate. Two of these peptides, PSBP(21-40) and PSBP(61-80), correspond to immunodominant self epitopes naturally processed in NOD mice after immunization with PSBP, whereas peptide PSBP(91-111) represents a cryptic epitope. These model systems address pathogenetic mechanisms in autoimmune prostatitis and will facilitate testing and mechanistic analysis of therapeutic approaches in this condition.
- Subjects :
- Amino Acid Sequence
Androgen-Binding Protein immunology
Androgen-Binding Protein metabolism
Animals
Antigen Presentation immunology
Autoantigens immunology
Autoantigens metabolism
Autoimmune Diseases metabolism
Autoimmune Diseases pathology
CD4-Positive T-Lymphocytes immunology
CD4-Positive T-Lymphocytes transplantation
Cell Movement immunology
Cells, Cultured
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred NOD
Mice, SCID
Molecular Sequence Data
Organ Specificity immunology
Peptide Fragments immunology
Peptide Fragments metabolism
Prostatein
Prostatitis metabolism
Prostatitis pathology
Rats
Self Tolerance immunology
Androgen-Binding Protein administration & dosage
Autoantigens administration & dosage
Autoimmune Diseases immunology
Peptide Fragments administration & dosage
Prostatitis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 179
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 17641022
- Full Text :
- https://doi.org/10.4049/jimmunol.179.3.1559