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[Change of splenocyte lymphokines in mice induced by recombinant BCG-Eg95 vaccine against Echinococcus granulosus].
- Source :
-
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases [Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi] 2007 Apr 30; Vol. 25 (2), pp. 109-13. - Publication Year :
- 2007
-
Abstract
- Objective: To investigate the reduction of hydatid cyst weight and change of splenocyte lymphokines in mice immunized with recombinant BCG-Eg95 vaccine of Echinococcus granulosus(Eg).<br />Methods: BALB/C mice were subcutaneously, intranasally, orally and intramuscularly vaccinated respectively, with BCG and PBS served as controls. The mice were challenged with Eg protoscolices 8 weeks after vaccination and sacrificed in 18 weeks after infection. The weight of hydatid cyst was measured and reduction of the weight was obtained, spleens were used to separate splenocytes which were cultured under stimulation with EgAg or ConA. The supernatant was collected to measure the level of IL-2, IFN-gamma, TNF-alpha and IL-4 by ELISA.<br />Results: The hydatid cyst weight reduced by 45.77%, 18.20%, 88.05% and 92.46% respectively in the 4 immunization groups. In comparison with PBS control, the level of IL-2, IFN-gamma and TNF-alpha was (30.0+/-0) pg/ml, (65.0+/-0) pg/ml and (425.0+/-10.7) pg/ml respectively in the intramuscular group with a significant increase, but that of IL-4 decreased, with a value of (10.0+/-0) pg/ml.<br />Conclusion: The recombinant BCG-Eg95 vaccination induces Thl response in mice against challenge infection.
- Subjects :
- Animals
Echinococcosis blood
Echinococcosis immunology
Echinococcosis parasitology
Female
Immunization methods
Interleukin-2 blood
Interleukin-4 blood
Mice
Mice, Inbred BALB C
Random Allocation
Spleen cytology
Spleen metabolism
Th1 Cells cytology
Th1 Cells immunology
Vaccines, Synthetic immunology
BCG Vaccine immunology
Echinococcus granulosus immunology
Lymphokines metabolism
Th1 Cells metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-7423
- Volume :
- 25
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
- Publication Type :
- Academic Journal
- Accession number :
- 17633820