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An improved sample preparation method for analyzing mycobacterial proteins in two-dimensional gels.
- Source :
-
Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2007 Jun; Vol. 72 (6), pp. 672-4. - Publication Year :
- 2007
-
Abstract
- Two-dimensional gel electrophoresis (2-DE) is currently a widely used analytical method for resolving complex mixtures of proteins. Sample preparation has a marked influence on 2-DE pattern. To reduce impurities and to increase the low-abundance proteins, protein precipitation is often used for the preparation of samples before 2-DE. In this study, we revealed that addition of SDS prior to TCA precipitation of mycobacterial cell extract proteins increases the resolution of the 2-D gel pattern.
Details
- Language :
- English
- ISSN :
- 0006-2979
- Volume :
- 72
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biochemistry. Biokhimiia
- Publication Type :
- Academic Journal
- Accession number :
- 17630913
- Full Text :
- https://doi.org/10.1134/s0006297907060119