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Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong.
- Source :
-
FEMS microbiology ecology [FEMS Microbiol Ecol] 2007 Sep; Vol. 61 (3), pp. 496-508. Date of Electronic Publication: 2007 Jul 11. - Publication Year :
- 2007
-
Abstract
- Diversity of particle-attached and free-living marine bacteria in Victoria Harbor, Hong Kong, and its adjacent coastal and estuarial environments was investigated using DNA fingerprinting and clone library analysis. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes showed that bacterial communities in three stations of Victoria Harbor were similar, but differed from those in adjacent coastal and estuarine stations. Particle-attached and free-living bacterial community composition differed in the Victoria Harbor area. DNA sequencing of 28 bands from DGGE gel showed Alphaproteobacteria was the most abundant group, followed by the Bacteroidetes, and other Proteobacteria. Bacterial species richness (number of DGGE bands) differed among stations and populations (particle-attached and free-living; bottom and surface). BIOENV analysis indicated that the concentrations of suspended solids were the major contributing parameter for the spatial variation of total bacterial community structure. Samples from representative stations were selected for clone library (548 clones) construction and their phylogenetic distributions were similar to those of sequences from DGGE. Approximately 80% of clones were affiliated to Proteobacteria, Bacteroidetes and Cyanobacteria. The possible influences of dynamic pollution and hydrological conditions in the Victoria Harbor area on the particle-attached and free-living bacterial community structures were discussed.
- Subjects :
- Alphaproteobacteria classification
Alphaproteobacteria genetics
Alphaproteobacteria ultrastructure
Bacteria classification
Bacteria ultrastructure
Bacteroidetes classification
Bacteroidetes genetics
Bacteroidetes ultrastructure
Cluster Analysis
Geography
Hong Kong
Microscopy, Electron, Scanning
Polymerase Chain Reaction
Proteobacteria classification
Proteobacteria genetics
Proteobacteria growth & development
RNA, Ribosomal, 16S genetics
Bacteria genetics
Ecosystem
Phylogeny
Seawater microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0168-6496
- Volume :
- 61
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- FEMS microbiology ecology
- Publication Type :
- Academic Journal
- Accession number :
- 17627779
- Full Text :
- https://doi.org/10.1111/j.1574-6941.2007.00353.x