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Interleukin-3 promotes the expression of E-NPP3/CD203C on human blood basophils in healthy subjects and in patients with birch pollen allergy.
- Source :
-
International journal of immunopathology and pharmacology [Int J Immunopathol Pharmacol] 2007 Apr-Jun; Vol. 20 (2), pp. 267-78. - Publication Year :
- 2007
-
Abstract
- We recently identified the ectoenzyme CD203c as a novel basophil activation antigen that is upregulated in response to FcepsilonRI cross-linkage. We investigated the effects of various interleukins (ILs) on expression of CD203c on blood basophils using an antibody against CD203c and flow cytometry. Of all cytokines tested, only IL-3 was found to upregulate expression of CD203c on basophils above baseline levels. The effects of IL-3 were dose- and time-dependent (EC(50): 0.1-1 ng/ml) without differences observed between healthy and allergic donors. Whereas anti-IgE induced maximum upregulation of CD203c within 15 minutes, the IL-3-induced upregulation showed a maximum after 180 minutes. IgE-receptor cross-linking resulted in enhanced expression of both CD63 and CD203c, whereas IL-3 enhanced the levels of CD203c without promoting expression of CD63. The IL-3-induced upregulation of CD203c was also observed in highly enriched basophils and was counteracted by a blocking antibody against the alpha chain of the IL-3 receptor (CD123). The IL-3-induced upregulation of CD203c was also found to depend on the presence of calcium. To analyze signaling pathways involved in IL-3-induced upregulation of CD203c, pharmacologic inhibitors were applied. The PI3-kinase inhibitors, wortmannin and LY294002 counteracted the IL-3-induced expression of CD203c, whereas MEK- and PKC inhibitors showed no effects. In conclusion, IL-3 upregulates expression of CD203c on basophils through a specific receptor and via a PI3-kinase-dependent signaling-pathway. Compared to FcepsilonRI-mediated cell activation, IL-3-induced upregulation of CD203c is a late(r) event and is not accompanied by upregulation of CD63.
- Subjects :
- Antigens, CD biosynthesis
Antigens, CD genetics
Cells, Cultured
Gene Expression Regulation physiology
Humans
Phosphoric Diester Hydrolases biosynthesis
Platelet Membrane Glycoproteins biosynthesis
Platelet Membrane Glycoproteins genetics
Pyrophosphatases biosynthesis
Tetraspanin 30
Basophils immunology
Basophils metabolism
Betula immunology
Interleukin-3 physiology
Phosphoric Diester Hydrolases genetics
Pollen immunology
Pyrophosphatases genetics
Rhinitis, Allergic, Seasonal immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0394-6320
- Volume :
- 20
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of immunopathology and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 17624239
- Full Text :
- https://doi.org/10.1177/039463200702000207