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Endobrevin/VAMP8 mediates exocytotic release of hexosaminidase from rat basophilic leukaemia cells.
- Source :
-
FEBS letters [FEBS Lett] 2007 Jul 24; Vol. 581 (18), pp. 3479-84. Date of Electronic Publication: 2007 Jun 29. - Publication Year :
- 2007
-
Abstract
- Mast cells are important players in innate immunity and mediate allergic responses. Upon stimulation, they release biologically active mediators including histamine, cytokines and lysosomal hydrolases. We used permeabilized rat basophilic leukaemia cells as model to identify R-SNAREs (soluble NSF (N-ethylmaleimide-sensitive fusion protein)) mediating exocytosis of hexosaminidase from mast cells. Of a complete set of recombinant mammalian R-SNAREs, only vesicle associated membrane protein (VAMP8)/endobrevin consistently blocked hexosaminidase release, which was also insensitive to treatment with clostridial neurotoxins. Thus, VAMP8, which also mediates fusion of late endosomes and lysosomes, plays a major role in hexosaminidase release, strengthening the view that mast cell granules share properties of both secretory granules and lysosomes.
- Subjects :
- Animals
Bacterial Proteins pharmacology
Basophils drug effects
Calcium pharmacology
Cell Line, Tumor
Cell Membrane Permeability drug effects
Leukemia genetics
R-SNARE Proteins genetics
Rats
Streptolysins pharmacology
Vesicle-Associated Membrane Protein 2 metabolism
Vesicle-Associated Membrane Protein 3 metabolism
Basophils metabolism
Exocytosis drug effects
Hexosaminidases metabolism
Leukemia metabolism
R-SNARE Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 581
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 17618625
- Full Text :
- https://doi.org/10.1016/j.febslet.2007.06.057