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The CREB leucine zipper regulates CREB phosphorylation, cardiomyopathy, and lethality in a transgenic model of heart failure.
- Source :
-
American journal of physiology. Heart and circulatory physiology [Am J Physiol Heart Circ Physiol] 2007 Sep; Vol. 293 (3), pp. H1877-82. Date of Electronic Publication: 2007 Jul 06. - Publication Year :
- 2007
-
Abstract
- Signaling through cAMP plays an important role in heart failure. Phosphorylation of cAMP response element binding protein (CREB) at serine-133 regulates gene expression in the heart. We examined the functional significance of CREB-S133 phosphorylation by comparing transgenic models in which a phosphorylation resistant CREB-S133A mutant containing either an intact or a mutated leucine zipper domain (CREB-S133A-LZ) was expressed in the heart. In vitro, CREB-S133A retained the ability to interact with wild-type CREB, whereas CREB-S133A-LZ did not. In vivo, CREB-S133A and CREB-S133A-LZ were expressed at comparable levels in the heart; however, CREB-S133A markedly suppressed the phosphorylation of endogenous CREB, whereas CREB-S133A-LZ had no effect. The one-year survival of mice from two CREB-S133A-LZ transgenic lines was equivalent to nontransgenic littermate control mice (NTG), whereas transgenic CREB-S133A mice died with heart failure at a median 30 wk of age (P < 0.0001). CREB-S133A mice had an altered gene expression characteristic of the failing heart, whereas CREB-S133A-LZ mice did not. Left ventricular contractile function was substantially reduced in CREB-S133A mice versus NTG mice and only modestly reduced in CREB-S133A-LZ mice (P < 0.02). When considered in light of other studies, these findings indicate that overexpression of the CREB leucine zipper is required for both inhibition of endogenous CREB phosphorylation and cardiomyopathy in this murine model of heart failure.
- Subjects :
- Animals
Cardiac Output, Low physiopathology
Cardiomyopathies physiopathology
Cyclic AMP Response Element-Binding Protein genetics
Disease Models, Animal
Female
Leucine Zippers genetics
Male
Mice
Mice, Transgenic
Mutation genetics
Myocardium metabolism
Phosphorylation
Protein Binding
Cardiac Output, Low metabolism
Cardiomyopathies metabolism
Cyclic AMP Response Element-Binding Protein metabolism
Leucine Zippers physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0363-6135
- Volume :
- 293
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Heart and circulatory physiology
- Publication Type :
- Academic Journal
- Accession number :
- 17616745
- Full Text :
- https://doi.org/10.1152/ajpheart.00516.2007