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Human maxillary tuberosity and jaw periosteum as sources of osteoprogenitor cells for tissue engineering.
Human maxillary tuberosity and jaw periosteum as sources of osteoprogenitor cells for tissue engineering.
- Source :
-
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics [Oral Surg Oral Med Oral Pathol Oral Radiol Endod] 2007 Nov; Vol. 104 (5), pp. 618.e1-12. Date of Electronic Publication: 2007 Jul 05. - Publication Year :
- 2007
-
Abstract
- Objective: Bone tissue engineering is a promising approach for bone reconstruction in oral-maxillofacial surgery. This study investigates the suitability of oral skeletal tissues as convenient and accessible sources of osteogenic progenitors as an alternative to the iliac crest bone marrow.<br />Study Design: Samples of maxilla tuberosity (MT) and maxillary and mandibular periosteum (MP) were obtained during routine oral surgery, and donor site morbidity was assessed using a "split-mouth" approach. Cells isolated from MT (bone marrow stromal cells; MT-BMSCs) and from MP (periosteal cells; M-PCs), were analyzed for clonogenicity, phenotype, expression of osteogenic markers, and ability to form bone in vivo.<br />Results: Both MT-BMSCs and M-PCs included clonogenic cells, showed comparable phenotypic profiles, and expressed early osteogenic markers. Most importantly, both cell populations formed bone upon ectopic in vivo transplantation.<br />Conclusion: MT-BMSCs and M-PCs behaved as osteoprogenitor cells in vitro and in vivo. MT and MP may be considered as suitable sources of cells for bone tissue engineering in humans.
- Subjects :
- ADP-ribosyl Cyclase metabolism
Adult
Analysis of Variance
Animals
Antigens, CD metabolism
Bone Regeneration
Cell Proliferation
Colony-Forming Units Assay methods
Female
Flow Cytometry methods
GPI-Linked Proteins
Humans
Male
Mice
Stem Cells cytology
Stem Cells metabolism
Transcription Factors analysis
Bone Marrow Cells cytology
Mandible cytology
Maxilla cytology
Periosteum cytology
Tissue Engineering methods
Subjects
Details
- Language :
- English
- ISSN :
- 1528-395X
- Volume :
- 104
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
- Publication Type :
- Academic Journal
- Accession number :
- 17613258
- Full Text :
- https://doi.org/10.1016/j.tripleo.2007.02.022