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Human Cdc45 is a proliferation-associated antigen.
- Source :
-
The FEBS journal [FEBS J] 2007 Jul; Vol. 274 (14), pp. 3669-3684. Date of Electronic Publication: 2007 Jul 03. - Publication Year :
- 2007
-
Abstract
- Cell division cycle protein 45 (Cdc45) plays a critical role in DNA replication to ensure that chromosomal DNA is replicated only once per cell cycle. We analysed the expression of human Cdc45 in proliferating and nonproliferating cells. Our findings show that Cdc45 protein is absent from long-term quiescent, terminally differentiated and senescent human cells, although it is present throughout the cell cycle of proliferating cells. Moreover, Cdc45 is much less abundant than the minichromosome maintenance (Mcm) proteins in human cells, supporting the concept that origin binding of Cdc45 is rate limiting for replication initiation. We also show that the Cdc45 protein level is consistently higher in human cancer-derived cells compared with primary human cells. Consequently, tumour tissue is preferentially stained using Cdc45-specific antibodies. Thus, Cdc45 expression is tightly associated with proliferating cell populations and Cdc45 seems to be a promising candidate for a novel proliferation marker in cancer cell biology.
- Subjects :
- Antigens genetics
Biomarkers, Tumor
Cell Cycle Proteins genetics
Cell Differentiation
Cell Line, Tumor
Cell Proliferation
Cellular Senescence physiology
Gene Expression Regulation
Humans
Immunohistochemistry
Ki-67 Antigen metabolism
Proliferating Cell Nuclear Antigen metabolism
Antigens immunology
Antigens metabolism
Cell Cycle Proteins immunology
Cell Cycle Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1742-464X
- Volume :
- 274
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- The FEBS journal
- Publication Type :
- Academic Journal
- Accession number :
- 17608804
- Full Text :
- https://doi.org/10.1111/j.1742-4658.2007.05900.x