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Identification of a fourth family of lycopene cyclases in photosynthetic bacteria.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2007 Jul 10; Vol. 104 (28), pp. 11784-9. Date of Electronic Publication: 2007 Jul 02. - Publication Year :
- 2007
-
Abstract
- A fourth and large family of lycopene cyclases was identified in photosynthetic prokaryotes. The first member of this family, encoded by the cruA gene of the green sulfur bacterium Chlorobium tepidum, was identified in a complementation assay with a lycopene-producing strain of Escherichia coli. Orthologs of cruA are found in all available green sulfur bacterial genomes and in all cyanobacterial genomes that lack genes encoding CrtL- or CrtY-type lycopene cyclases. The cyanobacterium Synechococcus sp. PCC 7002 has two homologs of CruA, denoted CruA and CruP, and both were shown to have lycopene cyclase activity. Although all characterized lycopene cyclases in plants are CrtL-type proteins, genes orthologous to cruP also occur in plant genomes. The CruA- and CruP-type carotenoid cyclases are members of the FixC dehydrogenase superfamily and are distantly related to CrtL- and CrtY-type lycopene cyclases. Identification of these cyclases fills a major gap in the carotenoid biosynthetic pathways of green sulfur bacteria and cyanobacteria.
- Subjects :
- Carotenoids biosynthesis
Chlorobium genetics
Escherichia coli genetics
Genetic Complementation Test
Intramolecular Lyases antagonists & inhibitors
Intramolecular Lyases genetics
Intramolecular Lyases metabolism
Lycopene
Molecular Sequence Data
Phylogeny
Synechococcus enzymology
Synechococcus genetics
Chlorobium enzymology
Intramolecular Lyases isolation & purification
Multigene Family
Photosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 104
- Issue :
- 28
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 17606904
- Full Text :
- https://doi.org/10.1073/pnas.0702984104