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The effect of the short wavelength ultraviolet radiation. An extension of biological dosimetry to the UV-C range.
- Source :
-
Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2007 Sep 25; Vol. 88 (2-3), pp. 77-82. Date of Electronic Publication: 2007 Feb 20. - Publication Year :
- 2007
-
Abstract
- Polycrystalline uracil thin layer can be used as biological dosimeter for assessing exposure to UV radiation. The dimerization and reversion efficiency of the ultraviolet radiation in the UV-B and the UV-C range were quantified on polycrystalline uracil thin layers irradiated with quasi-monochromatic radiation using interference filters of 10nm bandwidth. The dimer formation and monomerization (reversion) dose-effect relations were determined by optical spectroscopy. The decrease of the OD value of the uracil thin layer at 288 nm was taken as a measure of the dimer formation, while the increase of the OD of a completely irradiated (until reaching the saturation level) uracil layer was taken as the sign of the monomerization. The two processes in the UV-B and the UV-C range take place simultaneously, the individual characterization of the dimerization efficiency was performed from the initial slope of the dimerization dose-effect function and an action spectrum for dimerization was constructed in the UV-C range too. The reversion efficiency was found to be practically the same with all of the investigated wavelengths: 200 nm, 210 nm, 220 nm, 230 nm, 240 nm The possible biological relevance of the reversion of dimers are discussed.
Details
- Language :
- English
- ISSN :
- 1011-1344
- Volume :
- 88
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- Journal of photochemistry and photobiology. B, Biology
- Publication Type :
- Academic Journal
- Accession number :
- 17604638
- Full Text :
- https://doi.org/10.1016/j.jphotobiol.2007.02.001