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Mass spectrometric studies on mouse hippocampal synapsins Ia, IIa, and IIb and identification of a novel phosphorylation site at serine-546.
- Source :
-
Journal of proteome research [J Proteome Res] 2007 Jul; Vol. 6 (7), pp. 2695-710. Date of Electronic Publication: 2007 Jun 19. - Publication Year :
- 2007
-
Abstract
- Synapsins are key phosphoproteins in the mammalian brain, and structural research on synapsins is still holding center stage. Proteins were extracted from hippocampal tissue and separated on two-dimensional gel electrophoresis (2-DE), and the spots were analyzed by MALDI-TOF-TOF and nano-LC-ESI-MS/MS. Synapsins Ia, IIa, and IIb were unambiguously identified and represented by 15 individual spots on 2-DE. Several serine phosphorylation sites were confirmed, and a novel phosphorylation site was observed at Ser-546 in synapsin IIa in all gels analyzed.
- Subjects :
- Acetylation
Alternative Splicing
Amino Acid Sequence
Animals
Electrophoresis, Gel, Two-Dimensional
Male
Methionine chemistry
Methylation
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Nanotechnology methods
Phosphorylation
Protein Conformation
Protein Isoforms chemistry
Protein Isoforms genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
Synapsins genetics
Hippocampus chemistry
Phosphoproteins chemistry
Phosphoserine analysis
Serine chemistry
Synapsins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3893
- Volume :
- 6
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of proteome research
- Publication Type :
- Academic Journal
- Accession number :
- 17579389
- Full Text :
- https://doi.org/10.1021/pr070157r