Back to Search
Start Over
Heparin-binding EGF-like growth factor decreases neutrophil-endothelial cell interactions.
- Source :
-
The Journal of surgical research [J Surg Res] 2007 Aug; Vol. 141 (2), pp. 262-6. Date of Electronic Publication: 2007 Jun 14. - Publication Year :
- 2007
-
Abstract
- Background: Hyperadhesiveness of neutrophils (PMN) to vascular endothelial cells (EC) followed by neutrophil transendothelial migration play important roles in the initiation of ischemia/reperfusion (I/R)-mediated injury. We investigated whether the ability of heparin-binding EGF-like growth factor (HB-EGF) to decrease intestinal injury after intestinal I/R is mediated, in part, by its ability to affect PMN-EC interactions and EC junctional integrity.<br />Materials and Methods: Human umbilical vein EC monolayers were treated with HB-EGF (100 ng/mL) or phosphate-buffered saline followed by anoxia/reoxygenation (A/R). Simultaneously, labeled human PMN were treated with HB-EGF or phosphate-buffered saline and then co-incubated with EC for determination of PMN-EC adherence and PMN transendothelial migration. EC junctional integrity was also determined.<br />Results: PMN-EC adhesion increased after exposure of EC to A/R compared to EC exposed to normoxia (87% versus 64% binding, P < 0.05, Wilcoxon rank sum test). A/R-induced PMN-EC hyperadherence was significantly decreased by treatment of PMN with HB-EGF compared to nontreated cells (51% versus 87% binding, P < 0.05). HB-EGF significantly decreased PMN transendothelial migration and also augmented EC tight junctional integrity after A/R.<br />Conclusions: HB-EGF significantly reduces A/R-induced PMN-EC adhesion and PMN transendothelial migration and augments junctional integrity in vitro. Thus, HB-EGF acts not only as a potent cytoprotective agent for the intestine, but as an anti-inflammatory agent as well.
- Subjects :
- Adult
Cell Adhesion drug effects
Cell Movement drug effects
Cells, Cultured
Electric Impedance
Endothelial Cells physiology
Heparin-binding EGF-like Growth Factor
Humans
Intercellular Signaling Peptides and Proteins
Neutrophils physiology
Cell Communication drug effects
Endothelial Cells drug effects
Epidermal Growth Factor pharmacology
Neutrophils drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0022-4804
- Volume :
- 141
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of surgical research
- Publication Type :
- Academic Journal
- Accession number :
- 17574583
- Full Text :
- https://doi.org/10.1016/j.jss.2007.01.004