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Differential involvement of nitric oxide signaling in dopamine and PACAP stimulation of growth hormone release in goldfish.
- Source :
-
General and comparative endocrinology [Gen Comp Endocrinol] 2008 Jan 15; Vol. 155 (2), pp. 318-27. Date of Electronic Publication: 2007 May 13. - Publication Year :
- 2008
-
Abstract
- Previous studies in goldfish pituitary cells have shown that nitric oxide synthase (NOS)/nitric oxide (NO) signaling is involved in mediating the growth hormone (GH) release response to gonadotropin-releasing hormones. In this study, the involvement of this signaling pathway in mediating the action of two cAMP-mobilizing neuroendocrine stimulators of GH release, pituitary adenylate cyclase-activating polypeptide (PACAP) and dopamine (DA), was investigated in cell column perifusion experiments with primary cultures of dispersed pituitary cells. GH responses to PACAP were unaffected by three NOS inhibitors, aminoguanidine hemisulfate, 1400W and 7-nitroindazole (7-Ni). PACAP-stimulated GH release was also not reduced by two NO scavengers, rutin hydrate and PTIO, but NO-donor sodium nitroprusside (SNP)-elicited GH release was additive to the GH response to PACAP. In contrast, DA-induced GH secretion was reduced by 7-Ni, rutin hydrate and PTIO while not being additive to the GH response induced by SNP. These results indicate that although both PACAP and DA stimulation of acute GH release involve activation of adenylate cyclase/cAMP, DA- but not PACAP-signaling also utilizes the NOS/NO second messenger system.
- Subjects :
- Analysis of Variance
Animals
Cells, Cultured
Enzyme Inhibitors pharmacology
Fish Proteins metabolism
Nitric Oxide Synthase drug effects
Nitric Oxide Synthase metabolism
Pituitary Gland cytology
Pituitary Gland drug effects
Pituitary Gland metabolism
Signal Transduction physiology
Statistics, Nonparametric
Dopamine metabolism
Goldfish metabolism
Growth Hormone metabolism
Nitric Oxide physiology
Pituitary Adenylate Cyclase-Activating Polypeptide metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0016-6480
- Volume :
- 155
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- General and comparative endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 17574554
- Full Text :
- https://doi.org/10.1016/j.ygcen.2007.05.007