Back to Search
Start Over
Usefulness of Herpes Consensus PCR methodology to routine diagnostic testing for herpesviruses infections in clinical specimens.
- Source :
-
Virology journal [Virol J] 2007 Jun 12; Vol. 4, pp. 59. Date of Electronic Publication: 2007 Jun 12. - Publication Year :
- 2007
-
Abstract
- The purposes of the study were to assess the usefulness of simultaneously amplifying herpes simplex virus 1 and 2, varicella-zoster virus, cytomegalovirus, Epstein-Barr virus and human herpesvirus 6 DNA in various clinical specimens and to analyze clinical events in patients presenting positive results. A total of 763 clinical samples obtained from 758 patients, including 115 cerebrospinal fluids, 102 aqueous fluids, 445 swabs from genital (152), oro-facial (138) and other (155) skin lesions, 96 eye swabs and 5 bronchoalveolar lavages, were tested by using the Consensus polymerase chain reaction methodology. The clinical files of the patients were consulted retrospectively. 171 of the 758 patients (22.5%) were positive for at least one of the six target viruses: herpes simplex virus 1 (n = 95), varicella-zoster virus (n = 40), herpes simplex virus 2 (n = 21), herpes simplex virus 1 plus herpes simplex virus 2 (n = 8), cytomegalovirus (n = 4), Epstein-Barr virus (n = 1), human herpesvirus 6 (n = 1), and herpes simplex virus 1 plus human herpesvirus 6 (n = 1). The Consensus methodology enabled the rapid and accurate detection of herpesviruses in various clinical specimens and provided a reliable tool in the diagnosis of herpetic infections.
- Subjects :
- Adult
Bodily Secretions virology
Cerebrospinal Fluid virology
Child
Cytomegalovirus isolation & purification
DNA, Viral genetics
Herpesvirus 1, Human isolation & purification
Herpesvirus 2, Human isolation & purification
Herpesvirus 3, Human isolation & purification
Herpesvirus 4, Human isolation & purification
Herpesvirus 6, Human isolation & purification
Humans
Skin virology
Herpesviridae Infections diagnosis
Herpesviridae Infections virology
Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1743-422X
- Volume :
- 4
- Database :
- MEDLINE
- Journal :
- Virology journal
- Publication Type :
- Academic Journal
- Accession number :
- 17562023
- Full Text :
- https://doi.org/10.1186/1743-422X-4-59