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Peptidase profiles from non-albicans Candida spp. isolated from the blood of a patient with chronic myeloid leukemia and another with sickle cell disease.
- Source :
-
FEMS yeast research [FEMS Yeast Res] 2007 Sep; Vol. 7 (6), pp. 1004-12. Date of Electronic Publication: 2007 Jun 07. - Publication Year :
- 2007
-
Abstract
- Candida lipolytica and Candida rugosa were isolated from blood samples from a patient with chronic myeloid leukemia (31 years old) and a patient with sickle cell disease (1-year-old), respectively. Isolates were grown for 48 h at 37 degrees C in either Sabouraud or tryptone soy broth (TSB). Peptidases were tested for using substrate sodium dodecyl sulfate-polyacrylamide gels with gelatin, casein, bovine serum albumin (BSA) or hemoglobin. Enzymography analyses were made on the following substrates: human albumin, IgG and human fibrinogen, which had been incubated with the concentrated supernatants. For C. lipolytica, a approximately 60-kDa gelatin-degrading serine proteolytic activity was found in the TSB supernantant as well as a metallopeptidase activity capable of hydrolysing human albumin, IgG and human fibrinogen. With C. rugosa, albumin, IgG and human fibrinogen substrates were degraded by an aspartyl-like peptidase activity. Supernatants from C. rugosa also showed three serine proteolytic activities towards gelatin (approximately 50 kDa, TSB), casein ( approximately 94 kDa, TSB) and BSA ( approximately 120-kDa, Sabouraud), in addition to a metallopeptidase capable of degrading casein ( approximately 110 kDa, Sabouraud). Little is known about peptidases of C. rugosa and C. lipolytica and this preliminary data may prove useful for future work on host-parasite relationship and antifungal agents.
- Subjects :
- Adult
Blood Proteins metabolism
Candida drug effects
Candida albicans enzymology
Culture Media
Fungal Proteins chemistry
Fungal Proteins metabolism
Humans
Infant
Male
Protease Inhibitors pharmacology
Anemia, Sickle Cell complications
Candida enzymology
Candidiasis complications
Candidiasis microbiology
Leukemia, Myelogenous, Chronic, BCR-ABL Positive complications
Serine Endopeptidases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1567-1356
- Volume :
- 7
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- FEMS yeast research
- Publication Type :
- Academic Journal
- Accession number :
- 17559410
- Full Text :
- https://doi.org/10.1111/j.1567-1364.2007.00269.x