Back to Search Start Over

A tetrapeptide fragment-based design method results in highly stable artificial proteins.

A tetrapeptide fragment-based design method results in highly stable artificial proteins.

Authors :
Dallüge R
Oschmann J
Birkenmeier O
Lücke C
Lilie H
Rudolph R
Lange C
Source :
Proteins [Proteins] 2007 Sep 01; Vol. 68 (4), pp. 839-49.
Publication Year :
2007

Abstract

Computational protein design has progressed rapidly over the last years. A number of design methods have been proposed and tested. In this paper, we report the successful application of a fragment-based method for protein design. The method uses statistical information on tetrapeptide backbone conformations. The previously published artificial fold of TOP 7 (Kuhlman et al., Science, 2003; 302:1364-1368) was chosen as template. A series of polypeptide sequences were created that were predicted to fold into this target structure. Two of the designed proteins, M5 and M7, were expressed and characterized by fluorescence spectroscopy, circular dichroism and NMR. They showed the hallmarks of well-ordered tertiary structure as well as cooperative folding/unfolding transitions. Furthermore, the two novel proteins were found to be highly stable against temperature and denaturant-induced unfolding.<br /> (2007 Wiley-Liss, Inc.)

Details

Language :
English
ISSN :
1097-0134
Volume :
68
Issue :
4
Database :
MEDLINE
Journal :
Proteins
Publication Type :
Academic Journal
Accession number :
17557327
Full Text :
https://doi.org/10.1002/prot.21493