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Rescue of a chimeric rinderpest virus with the nucleocapsid protein derived from peste-des-petits-ruminants virus: use as a marker vaccine.
- Source :
-
The Journal of general virology [J Gen Virol] 2007 Jul; Vol. 88 (Pt 7), pp. 2019-2027. - Publication Year :
- 2007
-
Abstract
- The nucleocapsid (N) protein of all morbilliviruses has a highly conserved central region that is thought to interact with and encapsidate the viral RNA. The C-terminal third of the N protein is highly variable among morbilliviruses and is thought to be located on the outer surface and to be available to interact with other viral proteins such as the phosphoprotein, the polymerase protein and the matrix protein. Using reverse genetics, a chimeric rinderpest virus (RPV)/peste-des-petits-ruminants virus (PPRV) was rescued in which the RPV N gene open reading frame had been replaced with that of PPRV (RPV-PPRN). The chimeric virus maintained efficient replication in cell culture. Cattle vaccinated with this chimeric vaccine showed no adverse reaction and were protected from subsequent challenge with wild-type RPV, indicating it to be a safe and efficacious vaccine. The carboxyl-terminal variable region of the rinderpest N protein was cloned and expressed in Escherichia coli. The expressed protein was used to develop an indirect ELISA that could clearly differentiate between RPV- and PPRV-infected animals. The possibility of using this virus as a marker vaccine in association with a new diagnostic ELISA in the rinderpest eradication programme is discussed.
- Subjects :
- Animals
Antibodies, Viral analysis
Base Sequence
Cattle
Cattle Diseases immunology
Cattle Diseases prevention & control
Chimera genetics
Chimera immunology
Chlorocebus aethiops
Cloning, Molecular
DNA, Viral genetics
Diagnosis, Differential
Enzyme-Linked Immunosorbent Assay
Genes, Viral
Genetic Markers
Male
Peste-des-Petits-Ruminants diagnosis
Peste-des-Petits-Ruminants immunology
RNA, Viral genetics
RNA, Viral isolation & purification
Recombination, Genetic
Rinderpest diagnosis
Rinderpest immunology
Rinderpest prevention & control
Vero Cells
Viral Vaccines immunology
Virus Cultivation
Nucleocapsid Proteins genetics
Nucleocapsid Proteins immunology
Peste-des-petits-ruminants virus genetics
Peste-des-petits-ruminants virus immunology
Rinderpest virus genetics
Rinderpest virus immunology
Viral Vaccines genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1317
- Volume :
- 88
- Issue :
- Pt 7
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 17554036
- Full Text :
- https://doi.org/10.1099/vir.0.82913-0