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[The effect of interleukin-10 on neurocyte apoptosis in cerebral ischemia in rats].
- Source :
-
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2007 Jun; Vol. 23 (6), pp. 498-500. - Publication Year :
- 2007
-
Abstract
- Aim: To investigate the effect of interleukin-10 on neurocyte apoptosis in cerebral ischemia in rats.<br />Methods: 36 adult male Sprague-Dawley rats were randomly assigned into 3 groups: sham operation group, middle cerebral artery occlusion(MCAO) group and MCAO+interleukin-10 treatment group. Terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) staining was used to detect in situ cell apoptosis, immunohistochemical staining and RT-PCR were used to detect the expression of proapoptotic gene Fas, Fas ligand (FasL) and caspase-3 in peri-infarct, respectively.<br />Results: Cerebral ischemia significantly induced neurocyte apoptosis and upregulated the expression of Fas, FasL and caspase-3, repcetively (P<0.05). IL-10 treatment significantly inhibited neurocyte apoptosis, and suppressed the expression of proapoptotic gene FasL and caspase-3, repcetively (P<0.05), but had no obvious effect on Fas expression (P>0.05).<br />Conclusion: IL-10 can suppress neurocyte apoptosis in cerebral ischemia, whose mechanisms seemed related to inhibiting the expression of proapoptotic gene FasL and caspase-3.
- Subjects :
- Animals
Brain Ischemia metabolism
Caspase 3 genetics
Caspase 3 metabolism
Fas Ligand Protein genetics
Fas Ligand Protein metabolism
Immunohistochemistry
In Situ Nick-End Labeling
Male
Neurons cytology
Neurons metabolism
Random Allocation
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
fas Receptor genetics
fas Receptor metabolism
Apoptosis drug effects
Brain Ischemia pathology
Interleukin-10 pharmacology
Neurons drug effects
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1007-8738
- Volume :
- 23
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 17553341