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DNA bipyrimidine photoproduct repair and transcriptional response of UV-C irradiated Bacillus subtilis.
- Source :
-
Archives of microbiology [Arch Microbiol] 2007 Oct; Vol. 188 (4), pp. 421-31. Date of Electronic Publication: 2007 Jun 06. - Publication Year :
- 2007
-
Abstract
- Vegetative wild-type and DNA repair-deficient (homologous recombination, recA and nucleotide excision repair, uvrB) Bacillus subtilis cells were exposed to UV-C radiation. Colony formation, DNA bipyrimidine photoproducts and gene expression were measured during cell recovery. Gene expression was measured after 60 min cell recovery where 50% (wild-type), 30% (recA) and 8% (uvrB), respectively, of the UV-C induced DNA photoproducts were repaired. We examined changes in the gene expression following UV exposure in wild-type and both repair-deficient strains. A set of known and unknown genes were found to be significantly up-regulated in wild-type B. subtilis cells, whereas no or lower gene induction was determined for both mutant strains. In addition, the possible roles of newly identified UV-responsive genes are discussed with respect to cellular recovery following exposure to UV irradiation.
- Subjects :
- Bacillus subtilis growth & development
Colony Count, Microbial
DNA, Bacterial chemistry
Endodeoxyribonucleases genetics
Escherichia coli Proteins genetics
Gene Expression Profiling
Genes, Bacterial
Oligonucleotide Array Sequence Analysis
Pyrimidine Dimers analysis
Rec A Recombinases genetics
Transcriptional Activation
Bacillus subtilis genetics
Bacillus subtilis radiation effects
DNA Repair
Gene Expression Regulation, Bacterial
Ultraviolet Rays
Subjects
Details
- Language :
- English
- ISSN :
- 0302-8933
- Volume :
- 188
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Archives of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 17551712
- Full Text :
- https://doi.org/10.1007/s00203-007-0263-4