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Staphylococcus aureus RNAIII coordinately represses the synthesis of virulence factors and the transcription regulator Rot by an antisense mechanism.
- Source :
-
Genes & development [Genes Dev] 2007 Jun 01; Vol. 21 (11), pp. 1353-66. - Publication Year :
- 2007
-
Abstract
- RNAIII is the intracellular effector of the quorum-sensing system in Staphylococcus aureus. It is one of the largest regulatory RNAs (514 nucleotides long) that are known to control the expression of a large number of virulence genes. Here, we show that the 3' domain of RNAIII coordinately represses at the post-transcriptional level, the expression of mRNAs that encode a class of virulence factors that act early in the infection process. We demonstrate that the 3' domain acts primarily as an antisense RNA and rapidly anneals to these mRNAs, forming long RNA duplexes. The interaction between RNAIII and the mRNAs results in repression of translation initiation and triggers endoribonuclease III hydrolysis. These processes are followed by rapid depletion of the mRNA pool. In addition, we show that RNAIII and its 3' domain mediate translational repression of rot mRNA through a limited number of base pairings involving two loop-loop interactions. Since Rot is a transcriptional regulatory protein, we proposed that RNAIII indirectly acts on many downstream genes, resulting in the activation of the synthesis of several exoproteins. These data emphasize the multitude of regulatory steps affected by RNAIII and its 3' domain in establishing a network of S. aureus virulence factors.
- Subjects :
- 3' Untranslated Regions genetics
3' Untranslated Regions metabolism
Bacterial Proteins genetics
Base Sequence
Hydrolysis
Molecular Sequence Data
Nucleic Acid Conformation
Quorum Sensing
RNA, Antisense chemistry
RNA, Antisense genetics
RNA, Antisense metabolism
RNA, Bacterial chemistry
RNA, Bacterial genetics
RNA, Messenger genetics
RNA, Messenger metabolism
Sequence Homology, Nucleic Acid
Staphylococcus aureus genetics
Transcription, Genetic
Virulence Factors genetics
Bacterial Proteins metabolism
Gene Expression Regulation, Bacterial
RNA, Antisense pharmacology
RNA, Bacterial metabolism
Staphylococcus aureus enzymology
Virulence Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0890-9369
- Volume :
- 21
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Genes & development
- Publication Type :
- Academic Journal
- Accession number :
- 17545468
- Full Text :
- https://doi.org/10.1101/gad.423507