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[Targeted killing of colorectal tumor cells by lentiviral constructs containing CD/TK suicide genes and KDR promoter].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2007 May; Vol. 27 (5), pp. 624-7. - Publication Year :
- 2007
-
Abstract
- Objective: To investigate the selective killing of colorectal tumor cells by lentivirus-mediated double suicide gene under the regulation of KDR promoter.<br />Methods: 293T packaging cells were transfected with the plasmid FGW-KDRP-CD/TK to obtain the infectious viruses. KDR-expressing LoVo cells and LS174T cells that did not produce KDR were transfected with the recombinant virus, and the transfection efficiency was evaluated by the fluorecence microscope. RT-PCR was employed to examine the expression of CDglyTK. After treatment of the cells with 5-FC and GCV, the killing effects on the two cell lines were evaluated.<br />Results: The recombinant construct showed similar infection rate of the two cell lines. RT-PCR demonstrated that CDglyTK gene was expressed only in LoVo cells infected with FGW-KDRP-CD/TK but not in LS147T cells, and the sensitivity of the two cell lines to the prodrugs was significantly different (P<0.001). The killing effect of the double suicide gene was much stronger than that of single suicide gene administered (P<0.001).<br />Conclusion: The double suicide gene driven by KDR promoter has specific killing effect on the KDR-expressing colorectal tumor cells.
- Subjects :
- Antimetabolites pharmacology
Apoptosis drug effects
Cell Line
Cell Line, Tumor
Colorectal Neoplasms genetics
Colorectal Neoplasms metabolism
Colorectal Neoplasms pathology
Cytosine Deaminase genetics
Flow Cytometry
Flucytosine pharmacology
Ganciclovir pharmacology
Genetic Vectors genetics
Humans
Lentivirus genetics
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Thymidine Kinase genetics
Transfection
Vascular Endothelial Growth Factor Receptor-2 genetics
Cytosine Deaminase metabolism
Genes, Transgenic, Suicide genetics
Promoter Regions, Genetic genetics
Thymidine Kinase metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 27
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 17545072