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Effect of nitroxyl on the hamster retinal nitridergic pathway.
- Source :
-
Neurochemistry international [Neurochem Int] 2007 Nov-Dec; Vol. 51 (6-7), pp. 424-32. Date of Electronic Publication: 2007 May 03. - Publication Year :
- 2007
-
Abstract
- There is a growing body of evidence on the role of nitric oxide (NO) in retinal physiology. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO(-)), because it is formed by a number of diverse biochemical reactions. The aim of the present report was to comparatively analyze the effect of HNO and NO on the retinal nitridergic pathway in the golden hamster. For this purpose, sodium trioxodinitrate (Angeli's salt) and diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) were used as HNO and NO releasers, respectively. Angeli's salt and DEA/NO significantly decreased nitric oxide synthase activity. In addition, Angeli's salt (but not DEA/NO) significantly decreased l-arginine uptake. DEA/NO significantly increased cGMP accumulation at low micromolar concentrations, while Angeli's salt affected this parameter with a threshold concentration of 200muM. Although Angeli's salt and DEA/NO significantly diminished reduced glutathione and protein thiol levels in a similar way, DEA/NO was significantly more effective than AS in increasing S-nitrosothiol levels. None of these compounds increased retinal lipid peroxidation. These results suggest that HNO could regulate the hamster retinal nitridergic pathway by acting through a mechanism that only partly overlaps with that involved in NO response.
- Subjects :
- Animals
Antioxidants pharmacology
Arginine metabolism
Cricetinae
Cyclic GMP metabolism
Dose-Response Relationship, Drug
Glutathione drug effects
Glutathione metabolism
Lipid Peroxidation drug effects
Lipid Peroxidation physiology
Male
Mesocricetus
Nitric Oxide pharmacology
Nitric Oxide Synthase drug effects
Nitric Oxide Synthase metabolism
Nitrites pharmacology
Nitrogen Oxides pharmacology
Oxidative Stress drug effects
Oxidative Stress physiology
Quaternary Ammonium Compounds pharmacology
Retina drug effects
S-Nitrosothiols metabolism
Signal Transduction drug effects
Signal Transduction physiology
Visual Pathways drug effects
Nitrergic Neurons metabolism
Nitric Oxide metabolism
Nitrogen Oxides metabolism
Retina metabolism
Visual Pathways metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0197-0186
- Volume :
- 51
- Issue :
- 6-7
- Database :
- MEDLINE
- Journal :
- Neurochemistry international
- Publication Type :
- Academic Journal
- Accession number :
- 17543420
- Full Text :
- https://doi.org/10.1016/j.neuint.2007.04.015