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Involvement of chemical mediators in nasal allergic responses of HDC-KO mice.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2007 Jul 19; Vol. 567 (3), pp. 245-51. Date of Electronic Publication: 2007 Feb 08. - Publication Year :
- 2007
-
Abstract
- The present study was undertaken to investigate the involvement of chemical mediators in nasal allergic responses using histidine decarboxylase knockout (HDC-KO) mice. An allergic rhinitis model was developed in HDC-KO and wild-type mice by the intraperitoneal injection of ovalbumin, aluminum hydroxide gel and pertussis toxin. Five days later, they were boosted by a subcutaneous injection of ovalbumin into the back. From day 18 after the first immunization to day 39, intranasal sensitization with ovalbumin was performed every day and the severity of allergic rhinitis was observed by measuring nasal allergic responses and total IgE levels. It was found that the intranasal administration of antigen caused a significant increase of nasal sneezing and rubbing from day 25 to day 39 both in sensitized HDC-KO and wild-type mice. In addition, a significant elevation of total IgE levels in serum was also found both in sensitized HDC-KO and wild-type mice from day 18 to day 39 after the first immunization. L-733,060, a tachykinin NK(1) receptor antagonist at a dose of 10 mg/kg (s.c.), resulted in the dose-dependent inhibition of nasal allergic responses induced by antigen in both HDC-KO and wild-type mice. In addition, both chlorpheniramine at doses of 3 and 10 mg/kg (p.o.) and BW A868C at doses of 0.3 and 1 mg/kg (i.v.) also showed a dose-related reduction of the nasal allergic responses induced by antigen in sensitized wild-type mice. On the other hand, they had no effects on the nasal signs induced by antigen in HDC-KO mice. From these results, it was revealed that substance P induces nasal allergic responses in the mouse model of chronic allergic rhinitis through the activation of tachykinin NK(1) receptors. Therefore, it can be concluded that not only histamine, but also substance P and prostaglandin D(2), participated in the nasal allergic responses induced by antigen in mice.
- Subjects :
- Animals
Behavior, Animal physiology
Chlorpheniramine pharmacology
Histamine H1 Antagonists pharmacology
Histidine Decarboxylase deficiency
Hydantoins pharmacology
Immunization
Immunoglobulin E analysis
Immunoglobulin E immunology
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Neurokinin-1 Receptor Antagonists
Ovalbumin immunology
Piperidines pharmacology
Receptors, Immunologic antagonists & inhibitors
Receptors, Prostaglandin antagonists & inhibitors
Rhinitis, Allergic, Seasonal psychology
Sneezing physiology
Substance P pharmacology
Histidine Decarboxylase genetics
Histidine Decarboxylase physiology
Inflammation Mediators physiology
Rhinitis, Allergic, Seasonal physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2999
- Volume :
- 567
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 17543300
- Full Text :
- https://doi.org/10.1016/j.ejphar.2007.01.082