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Differential gene expression of aldolase C (ALDOC) and hypoxic adaptation in chickens.
- Source :
-
Animal genetics [Anim Genet] 2007 Jun; Vol. 38 (3), pp. 203-10. - Publication Year :
- 2007
-
Abstract
- Two sequence variants of the aldolase C (ALDOC) gene were discovered based on comparison of the sequences from an altiplano chicken breed (Tibetan chicken) and two lowland breeds (White Leghorn and ShouGuang). Gel-shift results indicated that one of these variants, L25375:c.310-258G>A, was able to bind hypoxia-induced factor-l (HIF1A), therefore, functioning as a hypoxia response element (HRE). The combined activity of the HRE and HIF1A could increase under the influence of a hypoxic stimulus. Hypoxia leads to increased death rates of chicken embryos, while the L25375:c.310-258G>A described herein is prevalent in healthy embryos grown under hypoxic conditions. Fluorescence quantitative real-time PCR results revealed that HIF1A upregulated the transcript level of the glycolytic enzyme ALDOC in the brain and skeletal muscle of animals subjected to hypoxia. Thus, a large amount of ATP is produced by increased glycolysis, allowing the organism to meet energy metabolism demands. As such, we believe this sequence variant is an adaptation to external anoxic environment.
- Subjects :
- Animals
Base Sequence
Brain metabolism
DNA Primers
Electrophoretic Mobility Shift Assay
Fructose-Bisphosphate Aldolase genetics
Molecular Sequence Data
Muscle, Skeletal metabolism
Oligonucleotides
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Adaptation, Physiological genetics
Chickens genetics
Fructose-Bisphosphate Aldolase metabolism
Gene Expression Regulation genetics
Hypoxia metabolism
Hypoxia-Inducible Factor 1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0268-9146
- Volume :
- 38
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Animal genetics
- Publication Type :
- Academic Journal
- Accession number :
- 17539972
- Full Text :
- https://doi.org/10.1111/j.1365-2052.2007.01605.x