[Effect of yishenqinghuo recipe on periodontal inflammation and immunity of rats with experimental periodontitis].

Purpose: To evaluate the effect of Yishenqinghuo recipe on periodontal inflammation and immunity of rats with experimental periodontitis.
Methods: 12 months old Spague-Dawley rats were used in this study. 78 rats were randomly divided into 4 groups. group A: control group (with no periodontitis, fed with the same dosage of saline as group D); group B: model group (with periodontitis, fed with the same dosage of saline as group D); group C: high dosage group (with periodontitis, fed with double dosage of medicine as group D); group D: equivalent dosage group (with periodontitis, fed with clinical equivalent effective dosage of medicine). After being gavaged with medicine/saline for 3 months, the periodontium was analyzed through histological slices; and the amount of CD4+T,CD8+T, the ratio of CD4+T/CD8+T, IL-2 and IL-1beta in peripheral blood were detected by flow cytometry and ELISA. All the results were analyzed by ANOVA, with the use of SAS 6.04 software package.
Results: It was found that the periodontal inflammation of group C and D were improved significantly; the ratio of CD4+T/CD8+T in peripheral blood for this 4 groups were 3.55 +/- 0.94, 2.42 +/- 0.75, 3.23 +/- 1.14 and 3.29 +/- 0.83; the level of IL-2 and IL-1beta were (36.03+/- 2.63/179.04 +/- 17.29) pg/ml, (25.18 +/- 3.08/306.09 +/- 13.38) pg/ml, (38.44 +/- 2.58/176.33 +/- 45.38) pg/ml and (36.81 +/- 2.45/182.13 +/- 43.97) pg/ml. Compared with group B, the ratio of CD4+T/CD8+T for group C and D was significantly rised (P < 1.05); the level of IL-2 increased significantlyand IL-1beta decreased accordingly (P < 0.01). However, there was no significant difference between group C and D (P > 0.05).
Conclusion: From this study, we conclude that Yishenqinghuo recipe can improve the periodontal inflammation and adjust the immunity of rats with experimental periodontitis. Supported by National "Tenth Five-Year" Key Science and Technology PROject (Grant No. 2004BA720A26) and Natural Science Foundation of Shanghai Municipality (Grant No. 03Zr14081).