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Chondrogenic differentiation of bovine synovium: bone morphogenetic proteins 2 and 7 and transforming growth factor beta1 induce the formation of different types of cartilaginous tissue.
- Source :
-
Arthritis and rheumatism [Arthritis Rheum] 2007 Jun; Vol. 56 (6), pp. 1869-79. - Publication Year :
- 2007
-
Abstract
- Objective: To compare the potential of bone morphogenetic proteins 2 and 7 (BMP-2 and BMP-7) and transforming growth factor beta1 (TGFbeta1) to effect the chondrogenic differentiation of synovial explants by analyzing the histologic, biochemical, and gene expression characteristics of the cartilaginous tissues formed.<br />Methods: Synovial explants derived from the metacarpal joints of calves were cultured in agarose. Initially, BMP-2 was used to evaluate the chondrogenic potential of the synovial explants under different culturing conditions. Under appropriate conditions, the chondrogenic effects of BMP-2, BMP-7, and TGFbeta1 were then compared. The differentiated tissue was characterized histologically, histomorphometrically, immunohistochemically, biochemically, and at the gene expression level.<br />Results: BMP-2 induced the chondrogenic differentiation of synovial explants in a dose- and time-dependent manner under serum- and dexamethasone-free conditions. The expression levels of cartilage-related genes increased in a time-dependent manner. BMP-7 was more potent than BMP-2 in inducing chondrogenesis, but the properties of the differentiated tissue were similar in each case. The type of cartilaginous tissue formed under the influence of TGFbeta1 differed in terms of both cell phenotype and gene expression profiles.<br />Conclusion: The 3 tested members of the TGFbeta superfamily have different chondrogenic potentials and induce the formation of different types of cartilaginous tissue. To effect the full differentiation of synovial explants into a typically hyaline type of articular cartilage, further refinement of the stimulation conditions is required. This might be achieved by the simultaneous application of several growth factors.
- Subjects :
- Aggrecans genetics
Aggrecans metabolism
Animals
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 7
Cartilage metabolism
Cartilage Oligomeric Matrix Protein
Cattle
Cells, Cultured
Chondrocytes metabolism
Collagen genetics
Collagen metabolism
Extracellular Matrix Proteins genetics
Extracellular Matrix Proteins metabolism
Glycoproteins genetics
Glycoproteins metabolism
Glycosaminoglycans genetics
Glycosaminoglycans metabolism
High Mobility Group Proteins genetics
High Mobility Group Proteins metabolism
Humans
Matrilin Proteins
Mesenchymal Stem Cells cytology
Mesenchymal Stem Cells metabolism
Recombinant Proteins
SOX9 Transcription Factor
Synovial Membrane metabolism
Transcription Factors genetics
Transcription Factors metabolism
Bone Morphogenetic Proteins physiology
Cartilage cytology
Cell Differentiation physiology
Chondrocytes cytology
Synovial Membrane cytology
Transforming Growth Factor beta physiology
Transforming Growth Factor beta1 physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0004-3591
- Volume :
- 56
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Arthritis and rheumatism
- Publication Type :
- Academic Journal
- Accession number :
- 17530715
- Full Text :
- https://doi.org/10.1002/art.22701