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Remote control of neuronal activity with a light-gated glutamate receptor.
- Source :
-
Neuron [Neuron] 2007 May 24; Vol. 54 (4), pp. 535-45. - Publication Year :
- 2007
-
Abstract
- The ability to stimulate select neurons in isolated tissue and in living animals is important for investigating their role in circuits and behavior. We show that the engineered light-gated ionotropic glutamate receptor (LiGluR), when introduced into neurons, enables remote control of their activity. Trains of action potentials are optimally evoked and extinguished by 380 nm and 500 nm light, respectively, while intermediate wavelengths provide graded control over the amplitude of depolarization. Light pulses of 1-5 ms in duration at approximately 380 nm trigger precisely timed action potentials and EPSP-like responses or can evoke sustained depolarizations that persist for minutes in the dark until extinguished by a short pulse of approximately 500 nm light. When introduced into sensory neurons in zebrafish larvae, activation of LiGluR reversibly blocks the escape response to touch. Our studies show that LiGluR provides robust control over neuronal activity, enabling the dissection and manipulation of neural circuitry in vivo.
- Subjects :
- Action Potentials physiology
Action Potentials radiation effects
Animals
Animals, Genetically Modified
Animals, Newborn
Behavior, Animal radiation effects
Cells, Cultured
Cysteine genetics
Dose-Response Relationship, Radiation
Electric Stimulation methods
Excitatory Postsynaptic Potentials
Hippocampus cytology
Larva
Leucine genetics
Mutation
Neurons drug effects
Neurons radiation effects
Patch-Clamp Techniques methods
Physical Stimulation methods
Rats
Receptors, Kainic Acid genetics
Transfection methods
Zebrafish
GluK2 Kainate Receptor
Behavior, Animal physiology
Lighting methods
Neurons physiology
Receptors, Kainic Acid physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0896-6273
- Volume :
- 54
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Neuron
- Publication Type :
- Academic Journal
- Accession number :
- 17521567
- Full Text :
- https://doi.org/10.1016/j.neuron.2007.05.010