Back to Search Start Over

Intimin subtyping of Escherichia coli: concomitant carriage of multiple intimin subtypes from forage-fed cattle and sheep.

Authors :
Cookson AL
Bennett J
Thomson-Carter F
Attwood GT
Source :
FEMS microbiology letters [FEMS Microbiol Lett] 2007 Jul; Vol. 272 (2), pp. 163-71. Date of Electronic Publication: 2007 May 22.
Publication Year :
2007

Abstract

The outer membrane protein, intimin (eae), which mediates bacterial attachment to epithelial cells, is associated with enteropathogenic Escherichia coli and some Shiga toxin-producing E. coli. The eae subtype of E. coli strains isolated from healthy cattle and sheep was identified using a rapid PCR-restriction fragment length polymorphism (RFLP) method to produce profiles that were compared with those generated in silico. The 139 eae-positive E. coli strains were separated into 11 different PCR-RFLP profiles. The most common eae PCR-RFLP type was beta (23.7%), followed by zeta (20.1%), theta (16.5%), iota (12.2%), kappa (8.6%), epsilon (7.2%), gamma (2.9%), nu and beta2 (2.2%) and iota2 (1.4%). Four isolates did not yield a PCR-RFLP amplification product but complete sequencing of the eae gene matched subtype rho. Two different eae variants were isolated from the same swab from 18 different animals and subtype iota was the most 'promiscuous', being isolated with four other eae subtypes from seven separate animals. None of the eae-positive STEC were subtype gamma, which is associated with STEC serogroup O157. This method allowed the rapid identification of eae subtypes and indicates that forage-fed animals possessed a wide diversity of bacterial eae subtypes with a low frequency of eae subtype gamma.

Details

Language :
English
ISSN :
0378-1097
Volume :
272
Issue :
2
Database :
MEDLINE
Journal :
FEMS microbiology letters
Publication Type :
Academic Journal
Accession number :
17521363
Full Text :
https://doi.org/10.1111/j.1574-6968.2007.00755.x