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Intimin subtyping of Escherichia coli: concomitant carriage of multiple intimin subtypes from forage-fed cattle and sheep.
- Source :
-
FEMS microbiology letters [FEMS Microbiol Lett] 2007 Jul; Vol. 272 (2), pp. 163-71. Date of Electronic Publication: 2007 May 22. - Publication Year :
- 2007
-
Abstract
- The outer membrane protein, intimin (eae), which mediates bacterial attachment to epithelial cells, is associated with enteropathogenic Escherichia coli and some Shiga toxin-producing E. coli. The eae subtype of E. coli strains isolated from healthy cattle and sheep was identified using a rapid PCR-restriction fragment length polymorphism (RFLP) method to produce profiles that were compared with those generated in silico. The 139 eae-positive E. coli strains were separated into 11 different PCR-RFLP profiles. The most common eae PCR-RFLP type was beta (23.7%), followed by zeta (20.1%), theta (16.5%), iota (12.2%), kappa (8.6%), epsilon (7.2%), gamma (2.9%), nu and beta2 (2.2%) and iota2 (1.4%). Four isolates did not yield a PCR-RFLP amplification product but complete sequencing of the eae gene matched subtype rho. Two different eae variants were isolated from the same swab from 18 different animals and subtype iota was the most 'promiscuous', being isolated with four other eae subtypes from seven separate animals. None of the eae-positive STEC were subtype gamma, which is associated with STEC serogroup O157. This method allowed the rapid identification of eae subtypes and indicates that forage-fed animals possessed a wide diversity of bacterial eae subtypes with a low frequency of eae subtype gamma.
- Subjects :
- Animals
Cattle
DNA, Bacterial chemistry
DNA, Bacterial genetics
Escherichia coli genetics
Escherichia coli Infections microbiology
Molecular Sequence Data
Polymerase Chain Reaction methods
Polymorphism, Restriction Fragment Length
Sequence Analysis, DNA
Sheep
Adhesins, Bacterial genetics
Bacterial Typing Techniques
Cattle Diseases microbiology
Escherichia coli classification
Escherichia coli Infections veterinary
Escherichia coli Proteins genetics
Sheep Diseases microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1097
- Volume :
- 272
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- FEMS microbiology letters
- Publication Type :
- Academic Journal
- Accession number :
- 17521363
- Full Text :
- https://doi.org/10.1111/j.1574-6968.2007.00755.x