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Epithelial cell kinetics of the gastric mucosa during Helicobacter pylori infection.
- Source :
-
FEMS immunology and medical microbiology [FEMS Immunol Med Microbiol] 2007 Jul; Vol. 50 (2), pp. 206-12. Date of Electronic Publication: 2007 May 22. - Publication Year :
- 2007
-
Abstract
- Helicobacter pylori is an important pathogen in major gastroduodenal diseases, including inflammation with ulceration and gastric malignancies. Alterations in H. pylori associated cell turnover in gastric epithelial cells are examined in relation to inflammatory activity, bacteria load and cytokines which may improve knowledge concerning the outcome of gastric diseases caused by H. pylori. Antral biopsies from 42 dyspeptic patients including 27 H. pylori-positive and 15 H. pylori-negative patients were tested for apoptotic activity by the TUNEL assay, and immuno-histochemically for p53 and the proliferative marker Ki-67. H. pylori infection, bacteria load and inflammatory activity were associated with increased cell turnover as judged by enhanced activities of TUNEL, p53 and Ki-67. Only p53 was significantly correlated to IFN-gamma, IL-8 and IL-10. The H. pylori-positive state was furthermore accompanied by varying degrees of altered distribution pattern of the markers studied, with occasional presence of apoptosis in the deeper pit zones, upward extension of Ki-67 and to a lesser degree of p53. Given a similar pattern of change in proliferation and apoptosis in some neoplastic lesions in other parts of the gastrointestinal tract, such studies in cell turnover may provide insights valuable in the investigations of potential precursors of gastric malignancies.
- Subjects :
- Apoptosis
Biopsy
Cell Proliferation
Female
Helicobacter Infections microbiology
Helicobacter pylori
Humans
Immunohistochemistry
In Situ Nick-End Labeling
Interferon-gamma biosynthesis
Interleukin-10 biosynthesis
Interleukin-8 biosynthesis
Ki-67 Antigen analysis
Kinetics
Male
Tumor Suppressor Protein p53 analysis
Epithelial Cells pathology
Gastric Mucosa pathology
Helicobacter Infections pathology
Pyloric Antrum pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0928-8244
- Volume :
- 50
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- FEMS immunology and medical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 17521356
- Full Text :
- https://doi.org/10.1111/j.1574-695X.2007.00254.x