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A heterologous prime-boost vaccination regime using DNA and a vaccinia virus, both expressing GRA4, induced protective immunity against Toxoplasma gondii infection in mice.

Authors :
Zhang G
Huong VT
Battur B
Zhou J
Zhang H
Liao M
Kawase O
Lee EG
Dautu G
Igarashi M
Nishikawa Y
Xuan X
Source :
Parasitology [Parasitology] 2007 Sep; Vol. 134 (Pt 10), pp. 1339-46. Date of Electronic Publication: 2007 May 17.
Publication Year :
2007

Abstract

SUMMARYThe dense granule antigen 4 (GRA4) is known as an immundominant antigen of Toxoplasma gondii and, therefore, is considered as a vaccine candidate. For further evaluation of its vaccine effect, a recombinant plasmid and vaccinia virus, both expressing GRA4, were constructed, and a heterologous prime-boost vaccination regime was performed in a mouse model. The mice immunized with the heterologous prime-boost vaccination regime showed a high level of specific antibody response against GRA4 and a significantly high level of gamma interferon (IFN-gamma) production and survived completely against a subsequent challenge infection with a lethal dose of T. gondii. In addition, the formation of cysts was inhibited in the mice vaccinated with the heterologous prime-boost vaccination regime. These results demonstrate that the heterologous prime-boost vaccination regime using DNA and a vaccinia virus, both expressing GRA4, could induce both humoral and cellular immune responses and provide effective protection against lethal acute and chronic T. gondii infections in mice.

Details

Language :
English
ISSN :
0031-1820
Volume :
134
Issue :
Pt 10
Database :
MEDLINE
Journal :
Parasitology
Publication Type :
Academic Journal
Accession number :
17506929
Full Text :
https://doi.org/10.1017/S0031182007002892