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A heterologous prime-boost vaccination regime using DNA and a vaccinia virus, both expressing GRA4, induced protective immunity against Toxoplasma gondii infection in mice.
- Source :
-
Parasitology [Parasitology] 2007 Sep; Vol. 134 (Pt 10), pp. 1339-46. Date of Electronic Publication: 2007 May 17. - Publication Year :
- 2007
-
Abstract
- SUMMARYThe dense granule antigen 4 (GRA4) is known as an immundominant antigen of Toxoplasma gondii and, therefore, is considered as a vaccine candidate. For further evaluation of its vaccine effect, a recombinant plasmid and vaccinia virus, both expressing GRA4, were constructed, and a heterologous prime-boost vaccination regime was performed in a mouse model. The mice immunized with the heterologous prime-boost vaccination regime showed a high level of specific antibody response against GRA4 and a significantly high level of gamma interferon (IFN-gamma) production and survived completely against a subsequent challenge infection with a lethal dose of T. gondii. In addition, the formation of cysts was inhibited in the mice vaccinated with the heterologous prime-boost vaccination regime. These results demonstrate that the heterologous prime-boost vaccination regime using DNA and a vaccinia virus, both expressing GRA4, could induce both humoral and cellular immune responses and provide effective protection against lethal acute and chronic T. gondii infections in mice.
- Subjects :
- Animals
Antibodies, Protozoan blood
Antibody Formation immunology
Brain parasitology
Chlorocebus aethiops
Female
Immunity, Cellular immunology
Interferon-gamma analysis
Mice
Mice, Inbred C57BL
Protozoan Proteins genetics
Time Factors
Toxoplasma genetics
Vero Cells
Protozoan Proteins immunology
Protozoan Vaccines immunology
Toxoplasma immunology
Toxoplasmosis immunology
Toxoplasmosis prevention & control
Vaccines, DNA immunology
Vaccinia virus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0031-1820
- Volume :
- 134
- Issue :
- Pt 10
- Database :
- MEDLINE
- Journal :
- Parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 17506929
- Full Text :
- https://doi.org/10.1017/S0031182007002892