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Excitatory GABA action is essential for morphological maturation of cortical neurons in vivo.
- Source :
-
The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2007 May 09; Vol. 27 (19), pp. 5224-35. - Publication Year :
- 2007
-
Abstract
- GABA exerts excitatory actions on embryonic and neonatal cortical neurons, but the in vivo function of this GABA excitation is essentially unknown. Using in utero electroporation, we eliminated the excitatory action of GABA in a subpopulation of rat ventricular progenitors and cortical neurons derived from these progenitors by premature expression of the Cl- transporter KCC2, as confirmed by the changes in the reversal potential of GABA-induced currents and the resting membrane potential after GABA(A) receptor blockade. We found that radial migration to layer II/III of the somatosensory cortex of neurons derived from the transfected progenitors was not significantly affected, but their morphological maturation was markedly impaired. Furthermore, reducing neuronal excitability of cortical neurons in vivo by overexpressing an inward-rectifying K+ channel, which lowered the resting membrane potential, mimicked the effect of premature KCC2 expression. Thus, membrane depolarization caused by early GABA excitation is critical for morphological maturation of neonatal cortical neurons in vivo.
- Subjects :
- Animals
Animals, Newborn
Bacterial Proteins genetics
Cell Communication physiology
Cell Membrane genetics
Cell Membrane metabolism
Cell Movement drug effects
Cell Movement genetics
Cerebral Cortex cytology
GABA Antagonists pharmacology
GABA-A Receptor Antagonists
Green Fluorescent Proteins genetics
Luminescent Proteins genetics
Membrane Potentials genetics
Neurons cytology
Organ Culture Techniques
Potassium Channels, Inwardly Rectifying genetics
Potassium Channels, Inwardly Rectifying metabolism
Rats
Rats, Sprague-Dawley
Receptors, GABA-A metabolism
Stem Cells metabolism
Symporters genetics
Symporters metabolism
Synaptic Transmission drug effects
gamma-Aminobutyric Acid pharmacology
K Cl- Cotransporters
Cell Differentiation physiology
Cerebral Cortex embryology
Cerebral Cortex growth & development
Neurons metabolism
Synaptic Transmission genetics
gamma-Aminobutyric Acid metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1529-2401
- Volume :
- 27
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- The Journal of neuroscience : the official journal of the Society for Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 17494709
- Full Text :
- https://doi.org/10.1523/JNEUROSCI.5169-06.2007