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Specific interactions of three proliferating cell nuclear antigens with replication-related proteins in Aeropyrum pernix.
- Source :
-
Molecular microbiology [Mol Microbiol] 2007 Apr; Vol. 64 (2), pp. 308-18. - Publication Year :
- 2007
-
Abstract
- Proliferating cell nuclear antigen (PCNA) is a well-known multifunctional protein involved in eukaryotic and archaeal DNA transactions. The homotrimeric PCNA ring encircles double-stranded DNA within its central hole and tethers many proteins on DNA. Plural genes encoding PCNA-like proteins have been found in the genome sequence of crenarchaeal organisms. We describe here the biochemical properties of the three PCNAs, PCNA1, PCNA2 and PCNA3, from the hyperthermophilic archaeon, Aeropyrum pernix. PCNA2 can form a trimeric structure by itself, and it also forms heterotrimeric structures with PCNA1 and PCNA3. However, neither PCNA1 nor PCNA3 can form homotrimers. The DNA synthesis activity of DNA polymerase I and II, the endonuclease activity of FEN1, and the nick-sealing activity of DNA ligase were stimulated by the complex of PCNA2 and 3 or PCNA1, 2 and 3. These results suggest that the heterotrimeric PCNA at least including PCNA2 and 3 function as the clamp in the replisome. However, PCNA2 is the most abundant in the cells throughout the growth stages among the three PCNAs, and therefore, PCNA2 may perform multitasks by changing complex composition.
- Subjects :
- Aeropyrum enzymology
Chromatography, Gel
DNA Cleavage
DNA Ligases metabolism
DNA Polymerase I metabolism
DNA Polymerase II metabolism
DNA Repair
DNA, Archaeal biosynthesis
Flap Endonucleases metabolism
Protein Binding
Protein Subunits metabolism
Replication Protein C metabolism
Aeropyrum metabolism
Archaeal Proteins metabolism
DNA Replication
Proliferating Cell Nuclear Antigen metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0950-382X
- Volume :
- 64
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 17493121
- Full Text :
- https://doi.org/10.1111/j.1365-2958.2007.05645.x