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The leukemoid reaction in Clostridium sordellii infection: neuraminidase induction of promyelocytic cell proliferation.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2007 Jun 15; Vol. 195 (12), pp. 1838-45. Date of Electronic Publication: 2007 May 02. - Publication Year :
- 2007
-
Abstract
- Life-threatening Clostridium sordellii infections have recently been reported in women undergoing therapeutic abortion, during natural childbirth, and in injection drug users. Shock, diffuse capillary leak, and a leukemoid reaction (LR) are cardinal features of these infections. The magnitude of the LR is highly correlated with mortality. We have isolated a 42-kDa extractable protein from C. sordellii culture supernatant that stimulates proliferation of promyelocytic HL-60 cells in vitro. Using mass spectrometry, we have identified this protein as the C. sordellii neuraminidase, NanS. Recombinant NanS (rNanS) dose dependently stimulated HL-60 cell proliferation. Increased proliferation was observed when HL-60 cells were cocultured with both rNanS and granulocyte-macrophage colony stimulating factor. In addition, NanS also modified vascular cell adhesion molecule 1, which orchestrates the release of mature and immature granulocytes from bone marrow stromal cells. Thus, neuraminidase likely plays an important role in the characteristic LR in C. sordellii infection.
- Subjects :
- Amino Acid Sequence
Cell Proliferation drug effects
Cloning, Molecular
Clostridium Infections microbiology
Clostridium sordellii genetics
DNA Primers chemistry
Dose-Response Relationship, Drug
Exotoxins pharmacology
Granulocyte-Macrophage Colony-Stimulating Factor pharmacology
HL-60 Cells
Humans
Leukemoid Reaction immunology
Leukemoid Reaction microbiology
Molecular Sequence Data
Neuraminidase biosynthesis
Neuraminidase genetics
Neuraminidase isolation & purification
Recombinant Proteins pharmacology
Time Factors
Vascular Cell Adhesion Molecule-1 drug effects
Clostridium Infections physiopathology
Clostridium sordellii enzymology
Granulocyte Precursor Cells drug effects
Leukemoid Reaction enzymology
Neuraminidase pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1899
- Volume :
- 195
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 17492600
- Full Text :
- https://doi.org/10.1086/518004