Back to Search
Start Over
[Homologous analysis of Streptococcus suis srt gene and prokaryotic expression of srtA].
- Source :
-
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2007 May; Vol. 23 (5), pp. 399-401. - Publication Year :
- 2007
-
Abstract
- Aim: To elucidate the distribution of srt gene in the genome of S.suis 05ZYH33, prokaryotically express Sortase A and analyze its antigenicity.<br />Methods: Homologous genes encoding sortase family members were analyzed, and then the srtA gene of S.suis was cloned and sequenced. The recombinant protein was analyzed by SDS-PAGE and Western blot. The mice were immunized with recombinant protein and the antibody titer was determined by indirect ELISA.<br />Results: All the putative srt genes in the genome of 05ZYH33 were analyzed. Six genes were found to be homologous to srt of S.suis isolated in Japan. The predicted srtA and sortase-like proteins were members of Class A of sortase family while srtBCD and srtE belonged to Class B. Western blot analysis showed that the recombinant protein was reactive to with the serum from the rabbits infected with a virulent strain of S.suis Type 2. The antibody titer in blood serum reached 1:6 400 after immunization with recombinant protein four times.<br />Conclusion: Compared with the isolated strain from Japan a new putative srt gene was found in 05ZYH33. The srtA gene was successfully expressed in prokaryotic system and the recombinant protein showed specific antigenicity, which is important for future research of the function of Sortase.
- Subjects :
- Aminoacyltransferases genetics
Aminoacyltransferases immunology
Aminoacyltransferases metabolism
Animals
Bacterial Proteins genetics
Bacterial Proteins immunology
Bacterial Proteins metabolism
Blotting, Western
Cysteine Endopeptidases genetics
Cysteine Endopeptidases immunology
Cysteine Endopeptidases metabolism
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Escherichia coli genetics
Escherichia coli metabolism
Genome, Bacterial genetics
Mice
Polymerase Chain Reaction
Recombinant Proteins immunology
Streptococcus suis immunology
Recombinant Proteins genetics
Recombinant Proteins metabolism
Streptococcus suis genetics
Streptococcus suis metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1007-8738
- Volume :
- 23
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 17488596